The Survivin Suppressant YM155 Potentiates Chemosensitivity to Gemcitabine in the Human Pancreatic Cancer Cell Line MiaPaCa-2

被引：0
|
作者：
Yoon, Dok Hyun [1 ,2 ]
Shin, Jae-Sik [1 ]
Jin, Dong-Hoon [1 ]
Hong, Seung-Woo [1 ]
Jung, Kyung A. [1 ]
Kim, Seung-Mi [1 ]
Hong, Yong Sang [1 ,2 ]
Kim, Kyu-Pyo [1 ,2 ]
Lee, Jae-Lyun [1 ,2 ]
Suh, Cheolwon [2 ]
Lee, Jung Shin [1 ,2 ]
Kim, Tae Won [1 ,2 ]
机构：
[1] Univ Ulsan, Coll Med, Inst Innovat Canc Res, Asan Med Ctr, Seoul 138736, South Korea
[2] Univ Ulsan, Coll Med, Dept Oncol, Asan Med Ctr, Seoul 138736, South Korea
来源：
ANTICANCER RESEARCH | 2012年 / 32卷 / 05期
关键词：
Pancreatic cancer; survivin; YM155; gemcitabine; MiaPaCa-2; in vivo model; xenograft; GENE; GROWTH; ADENOCARCINOMA; INHIBITION; EXPRESSION; MUTATIONS; APOPTOSIS; MELANOMA; TUMORS; TRIAL;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Survivin is a negative regulator of apoptosis. We evaluated the efficacy of YM155, a selective suppressant of survivin, in combination with gemcitabine in the pancreatic cancer cell line MiaPaCa-2. Materials and Methods: Expression of survivin was demonstrated by immunoblotting. Cell cycle progression was determined by flow cytometric analysis. Cell viability was assayed using the trypan blue exclusion assay. Results: Gemcitabine up-regulated survival expression, whereas treatment with YM155 suppressed the expression of survivin. Concomitant treatment with YM155 enhanced chemosensitivity to gemcitabine, which was accompanied by a decrease in the expression of survivin. Knockdown of endogenous survivin via RNA interference also enhanced the sensitivity to gemcitabine. In addition, YM155 potentiated the antitumor effect of gemcitabine in xenograft tumors of MiaPaCa-2. Conclusion: YM155 potentiates chemosensitivity to gemcitabine in pancreatic cancer cells by suppressing the induction of survival. Combination treatment with gemcitabine and YM155 may be a potential therapeutic strategy for the treatment of pancreatic cancer that warrants further clinical investigation.
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页码：1681 / 1688
页数：8
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