Aedes aegypti D7 Saliva Protein Inhibits Dengue Virus Infection

被引：64

作者：

Conway, Michael J. ^{[1
]}

Londono-Renteria, Berlin ^{[2
]}

Troupin, Andrea ^{[2
]}

Watson, Alan M. ^{[3
,4
]}

Klimstra, William B. ^{[3
,4
]}

Fikrig, Erol ^{[5
,6
]}

Colpitts, Tonya M. ^{[2
]}

机构：

[1] Cent Michigan Univ, Coll Med, Fdn Sci, Mt Pleasant, MI 48859 USA

[2] Univ South Carolina, Sch Med, Dept Pathol Microbiol & Immunol, Columbia, SC USA

[3] Univ Pittsburgh, Ctr Vaccine Res, Pittsburgh, PA USA

[4] Univ Pittsburgh, Dept Microbiol & Mol Genet, Pittsburgh, PA USA

[5] Yale Univ, Sch Med, Dept Internal Med, Infect Dis Sect, New Haven, CT 06510 USA

[6] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2016年 / 10卷 / 09期

关键词：

MOSQUITO SALIVA; ARBOVIRUS TRANSMISSION; FEMALE MOSQUITO; MICE; IGE; POTENTIATION; ANTIBODIES; EXTRACTS; DISEASE; BINDING;

D O I：

10.1371/journal.pntd.0004941

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV) types 1-4. Ae. aegypti transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. Ae. aegypti saliva contains over one hundred unique proteins and these proteins have diverse functions, including facilitating blood feeding. Previously, we showed that Ae. aegypti salivary gland extracts (SGEs) enhanced dissemination of DENV to draining lymph nodes. In contrast, HPLC-fractionation revealed that some SGE components inhibited infection. Here, we show that D7 proteins are enriched in HPLC fractions that are inhibitory to DENV infection, and that recombinant D7 protein can inhibit DENV infection in vitro and in vivo. Further, binding assays indicate that D7 protein can directly interact with DENV virions and recombinant DENV envelope protein. These data reveal a novel role for D7 proteins, which inhibits arbovirus transmission to vertebrates through a direct interaction with virions.

引用

页数：19

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