Intra-host evolutionary dynamics of hepatitis C virus E2 in treated patients

被引:16
作者
Alfonso, V
Mbayed, VA
Sookoian, S
Campos, RH
机构
[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Virol, RA-1113 Buenos Aires, DF, Argentina
[2] Hosp Argerich, Unidad Hepatol, Buenos Aires, DF, Argentina
关键词
D O I
10.1099/vir.0.81084-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Hepatitis C virus (HCV) displays high genetic diversity. Inter-host sequence variability may mainly reflect a neutral drift evolution. In contrast, intra-host evolution may be driven by an adaptive selection to host responses to infection. Here, HCV E2 intra-host evolution in two patients during the course and follow-up of successive treatments with IFN-alpha and IFN-alpha/ribavirin was investigated. Phylogenetic analyses suggested that adaptive pressures prompt a continuous selection of viral variants derived from the previous ones (intra-lineage evolution) and/or a swapping of viral lineages during the course of the infection (inter-lineage evolution). Selection would act not only on the phenotypic features of hypervariable, region 1 (HVR1) but also on those of the flanking regions. The pressures operate mainly at the amino acid level, but they also appeared to act on nucleotide sequences. Moreover, HVR1 heterogeneity seemed to be strongly constrained. This work contributes to the knowledge of HCV intra-host evolution during chronicity.
引用
收藏
页码:2781 / 2786
页数:6
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