Comprehensive analysis of the ceRNA network in coronary artery disease

被引：13

作者：

Bian, Weikang ^{[1
]}

Jiang, Xiao-Xin ^{[1
]}

Wang, Zhicheng ^{[1
]}

Zhu, Yan-Rong ^{[1
]}

Zhang, Hongsong ^{[1
]}

Li, Xiaobo ^{[1
]}

Liu, Zhizhong ^{[1
]}

Xiong, Jing ^{[2
]}

Zhang, Dai-Min ^{[1
]}

机构：

[1] Nanjing Med Univ, Nanjing First Hosp, Dept Cardiol, 68 Changle Rd, Nanjing 210006, Jiangsu, Peoples R China

[2] China Pharmaceut Univ, Dept Pharmacol, Nanjing 210009, Jiangsu, Peoples R China

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

基金：

中国国家自然科学基金;

关键词：

LONG NONCODING RNA; H19; EXPRESSION; DATABASE; INFLAMMATION; POLYMORPHISM; PREDICTION; BIOMARKERS; MICRORNAS; PROGNOSIS;

D O I：

10.1038/s41598-021-03688-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

With the rapid aging of the population, coronary artery disease (CAD) has become one of the most fatal chronic diseases. However, the genetic mechanism of CAD is still unclear. The purpose of this study is to construct the lncRNA-miRNA-mRNA regulatory network for CAD diseases and systematically identify differentially expressed genes in patients with coronary heart disease. In this study, two lncRNA datasets (GSE69587 and GSE113079) and a microRNA dataset (GSE105449) which contained 393 and 38 CAD samples were selected. In addition, two mRNA datasets which named GSE113079 (98 CAD samples) and GSE9820 (8 CAD samples) were selected to search the differentially expressed genes (DEGs). By comparing the expression data between CAD and control samples, a total of 1111 lncRNAs, 2595 mRNAs and 22 miRNAs were identified. Based on the DEGs, a lncRNA-miRNA-mRNA ceRNA network was constructed to explore the hub nodes in CAD. In the ceRNA network, the lncRNAs KCNQ1OT1 and H19 showed high connectivity with the nine miRNAs. GO and KEGG results showed that genes in ceRNA networks were mainly involved in nitrogen compound metabolic process, PI3K-Akt signaling pathway and retrograde endocannabinoid signaling. These findings will improve the understanding of the occurrence and development mechanism of CAD.

引用

页数：11

共 51 条

[1] Circulating microRNAs: New biomarkers in diagnosis, prognosis and treatment of cancer (Review) [J].

Allegra, Alessandro ;

Alonci, Andrea ;

Campo, Salvatore ;

Penna, Giuseppa ;

Petrungaro, Annamaria ;

Gerace, Demetrio ;

Musolino, Caterina .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2012, 41 (06) :1897-1912

[2] MicroRNA-21 Promotes Fibrosis of the Kidney by Silencing Metabolic Pathways [J].

Chau, B. Nelson ;

Xin, Cuiyan ;

Hartner, Jochen ;

Ren, Shuyu ;

Castano, Ana P. ;

Linn, Geoffrey ;

Li, Jian ;

Tran, Phong T. ;

Kaimal, Vivek ;

Huang, Xinqiang ;

Chang, Aaron N. ;

Li, Shenyang ;

Kalra, Aarti ;

Grafals, Monica ;

Portilla, Didier ;

MacKenna, Deidre A. ;

Orkin, Stuart H. ;

Duffield, Jeremy S. .

SCIENCE TRANSLATIONAL MEDICINE, 2012, 4 (121)

[3] Predicting lncRNA-disease associations and constructing lncRNA functional similarity network based on the information of miRNA [J].

Chen, Xing .

SCIENTIFIC REPORTS, 2015, 5

[4] cytoHubba: identifying hub objects and sub-networks from complex interactome [J].

Chin, Chia-Hao ;

Chen, Shu-Hwa ;

Wu, Hsin-Hung ;

Ho, Chin-Wen ;

Ko, Ming-Tat ;

Lin, Chung-Yen .

BMC SYSTEMS BIOLOGY, 2014, 8

[5]

Clough E, 2016, METHODS MOL BIOL, V1418, P93, DOI 10.1007/978-1-4939-3578-9_5

[6] CD90+liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA [J].

Conigliaro, Alice ;

Costa, Viviana ;

Lo Dico, Alessia ;

Saieva, Laura ;

Buccheri, Simona ;

Dieli, Francesco ;

Manno, Mauro ;

Raccosta, Samuele ;

Mancone, Carmine ;

Tripodi, Marco ;

De Leo, Giacomo ;

Alessandro, Riccardo .

MOLECULAR CANCER, 2015, 14

[7]

Cui JD, 2015, INT J CLIN EXP PATHO, V8, P12400

[8] Leukocyte-Specific CCL3 Deficiency Inhibits Atherosclerotic Lesion Development by Affecting Neutrophil Accumulation [J].

de Jager, Saskia C. A. ;

Bot, Ilze ;

Kraaijeveld, Adriaan O. ;

Korporaal, Suzanne J. A. ;

Bot, Martine ;

van Santbrink, Peter J. ;

van Berkel, Theo J. C. ;

Kuiper, Johan ;

Biessen, Erik A. L. .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2013, 33 (03) :E75-+

[9]

Edris B., 2011, BMI231

[10] Small molecules with big roles in microRNA chemical biology and microRNA-targeted therapeutics [J].

Fan, Rengen ;

Xiao, Chaocheng ;

Wan, Xinqiang ;

Cha, Wenzhang ;

Miao, Yufeng ;

Zhou, Yong ;

Qin, Chenglin ;

Cui, Ting ;

Su, Fenglian ;

Shan, Xiangxiang .

RNA BIOLOGY, 2019, 16 (06) :707-718

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