Upregulation of an Artificial Zymogen by Proteolysis

被引：31

作者：

Liu, Zhe ^{[1
,4
]}

Lebrun, Vincent ^{[1
]}

Kitanosono, Taku ^{[2
]}

Mallin, Hendrik ^{[1
]}

Kohler, Valentin ^{[1
]}

Haussinger, Daniel ^{[1
]}

Hilvert, Donald ^{[3
]}

Kobayashi, Shu ^{[2
]}

Ward, Thomas R. ^{[1
]}

机构：

[1] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

[2] Univ Tokyo, Dept Chem, Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

[3] ETH, Organ Chem Lab, CH-8093 Zurich, Switzerland

[4] Qufu Normal Univ, Sch Chem & Chem Engn, Qufu 273165, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2016年 / 55卷 / 38期

基金：

瑞士国家科学基金会;

关键词：

artificial metalloenzyme; artificial zymogen; asymmetric catalysis; transfer hydrogenation; upregulation; STREPTAVIDIN; METALLOENZYMES; PROTEIN; HYDROGENATION; CATALYSIS; COMPLEXES; DESIGN;

D O I：

10.1002/anie.201605010

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Regulation of enzymatic activity is vital to living organisms. Here, we report the development and the genetic optimization of an artificial zymogen requiring the action of a natural protease to upregulate its latent asymmetric transfer hydrogenase activity.

引用

页码：11587 / 11590

页数：4

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