Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses

被引：170

作者：

Bueckers, Johanna ^{[1
]}

Wildanger, Dominik ^{[1
]}

Vicidomini, Giuseppe ^{[1
]}

Kastrup, Lars ^{[1
]}

Hell, Stefan W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

来源：

OPTICS EXPRESS | 2011年 / 19卷 / 04期

关键词：

BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STIMULATED-EMISSION; CHANNEL SEPARATION; LIVING CELLS; MICROSCOPY; NANOSCOPY; LOCALIZATION; RESOLUTION; PROBES; VISUALIZATION;

D O I：

10.1364/OE.19.003130

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording. (C) 2011 Optical Society of America

引用

页码：3130 / 3143

页数：14

共 30 条

[1]

[Anonymous], 1930, The Physical Principles of Quantum Theory

[2] Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging [J].

Auksorius, Egidijus ;

Boruah, Bosanta R. ;

Dunsby, Christopher ;

Lanigan, Peter M. P. ;

Kennedy, Gordon ;

Neil, Mark A. A. ;

French, Paul M. W. .

OPTICS LETTERS, 2008, 33 (02) :113-115

[3] Multicolor super-resolution imaging with photo-switchable fluorescent probes [J].

Bates, Mark ;

Huang, Bo ;

Dempsey, Graham T. ;

Zhuang, Xiaowei .

SCIENCE, 2007, 317 (5845) :1749-1753

[4] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[5] Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species [J].

Bossi, Mariano ;

Foelling, Jonas ;

Belov, Vladimir N. ;

Boyarskiy, Vadim P. ;

Medda, Rebecca ;

Egner, Alexander ;

Eggeling, Christian ;

Schoenle, Andreas ;

Hell, Stefan W. .

NANO LETTERS, 2008, 8 (08) :2463-2468

[6] SIMULTANEOUS CONFOCAL RECORDING OF MULTIPLE FLUORESCENT LABELS WITH IMPROVED CHANNEL SEPARATION [J].

CARLSSON, K ;

ASLUND, N ;

MOSSBERG, K ;

PHILIP, J .

JOURNAL OF MICROSCOPY-OXFORD, 1994, 176 :287-299

[7] Confocal fluorescence microscopy using spectral and lifetime information to simultaneously record four fluorophores with high channel separation [J].

Carlsson, K ;

Liljeborg, A .

JOURNAL OF MICROSCOPY, 1997, 185 :37-46

[8] Two-color far-field fluorescence nanoscopy [J].

Donnert, Gerald ;

Keller, Jan ;

Wurm, Christian A. ;

Rizzoli, Silvio O. ;

Westphal, Volker ;

Schoenle, Andreas ;

Jahn, Reinhard ;

Jakobs, Stefan ;

Eggeling, Christian ;

Hell, Stefan W. .

BIOPHYSICAL JOURNAL, 2007, 92 (08) :L67-L69

[9] Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters [J].

Egner, Alexander ;

Geisler, Claudia ;

Von Middendorff, Claas ;

Bock, Hannes ;

Wenzel, Dirk ;

Medda, Rebecca ;

Andresen, Martin ;

Stiel, Andre C. ;

Jakobs, Stefan ;

Eggeling, Christian ;

Schoenle, Andreas ;

Hell, Stefan W. .

BIOPHYSICAL JOURNAL, 2007, 93 (09) :3285-3290

[10]

ENGELHARDT J, 2010, NANO LETT

← 1 2 3 →