Enhanced initial cell responses to chemically modified anodized titanium

被引：59

作者：

Jimbo, Ryo ^{[1
]}

Sawase, Takashi ^{[1
]}

Baba, Koumei ^{[2
]}

Kurogi, Tadafumi ^{[3
]}

Shibata, Yasuaki ^{[4
]}

Atsuta, Mitsuru ^{[1
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Div Appl Prosthodont, Nagasaki 8528588, Japan

[2] Ind Technol Ctr, Nagasaki, Japan

[3] Nagasaki Univ, Grad Sch Biomed Sci, Div Prosthet Dent, Nagasaki 8528588, Japan

[4] Nagasaki Univ, Grad Sch Biomed Sci, Div Oral Pathol & Bone Metab, Nagasaki 8528588, Japan

来源：

CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH | 2008年 / 10卷 / 01期

关键词：

ammonium hydrogen fluoride; anodization; hydrophilicity; titanium; ultraviolet irradiation;

D O I：

10.1111/j.1708-8208.2007.00061.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Background: Previously, we reported that anodized porous titanium implants have photocatalytic hydrophilicity. However, this effect was not always sufficient for the significant improvement of bone apposition. Purpose: The purpose of this study was to improve the photocatalytic properties of porous titanium implants by the fluoride modification of the anodized titanium dioxide (TiO2), and to investigate the initial cell response to it. Materials and Methods: The ideal concentration of ammonium hydrogen fluoride (NH4F-HF2) used in this study was determined by a static water contact angle assay. The ideal concentration of NH4F-HF2 was 0.175%, and experimental disks were treated with this concentration. A pluripotent mesenchymal cell line, C2C12, was cultured on the disks in order to investigate cell attachment, morphology, and proliferation. Results: Cell attachment after 30 minutes of culturing was significantly higher for the ultraviolet-irradiated, fluoride-modified anodized TiO2 (p < .05), and the simultaneous scanning electron microscope observation showed a rather flattened and extended cell morphology. The proliferation rate after 24 hours was also significantly higher for the fluoride-modified anodized TiO2. Conclusion: Fluoride chemical modification enhances the hydrophilic property of the anodized TiO2 and improves the initial cell response to it.

引用

页码：55 / 61

页数：7

共 26 条

[11] High photocatalytic activity of F-doped TiO2 film on glass [J].

Hattori, A ;

Tada, H .

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2001, 22 (1-2) :47-52

[12]

KASSEM M, 1993, J BONE MINER RES, V8, P1453

[13] EFFECTS OF FLUORIDE ON HUMAN BONE-CELLS IN-VITRO - DIFFERENCES IN RESPONSIVENESS BETWEEN STROMAL OSTEOBLAST PRECURSORS AND MATURE OSTEOBLASTS [J].

KASSEM, M ;

MOSEKILDE, L ;

ERIKSEN, EF .

EUROPEAN JOURNAL OF ENDOCRINOLOGY, 1994, 130 (04) :381-386

[14]

Kilpadi KL, 2001, J BIOMED MATER RES, V57, P258, DOI 10.1002/1097-4636(200111)57:2<258::AID-JBM1166>3.0.CO

[15]

2-R

[16] A comparative study of supported TiO2 as photocatalyst in water decontamination at solar pilot plant scale [J].

Kus, Mahmut ;

Gernjak, Wolfgang ;

Fernandez Ibanez, Pilar ;

Malato Rodriguez, Sixto ;

Blanco Galvez, Julian ;

Icli, Siddik .

JOURNAL OF SOLAR ENERGY ENGINEERING-TRANSACTIONS OF THE ASME, 2006, 128 (03) :331-337

[17] Photocatalytic and electrochemically assisted photocatalytic oxidation of formic acid on TiO2 films under UVA and UVB irradiation [J].

McMurray, TA ;

Byrne, JA ;

Dunlop, PSM ;

McAdams, ET .

JOURNAL OF APPLIED ELECTROCHEMISTRY, 2005, 35 (7-8) :723-731

[18] Effect of repeated photo-illumination on the wettability conversion of titanium dioxide [J].

Nakajima, A ;

Koizumi, S ;

Watanabe, T ;

Hashimoto, K .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2001, 146 (1-2) :129-132

[19]

NAKAMURA H, 2006, IN PRESS BIOSENS BIO

[20] Roughness induced dynamic changes of wettability of acid etched titanium implant modifications [J].

Rupp, F ;

Scheideler, L ;

Rehbein, D ;

Axmann, D ;

Gels-Gerstorfer, J .

BIOMATERIALS, 2004, 25 (7-8) :1429-1438

← 1 2 3 →