Production, characterization and application of thermostable, alkaline α-amylase (AA11) from Bacillus cereus strain SP-CH11 isolated from Chilika Lake

被引:18
作者
Priyadarshini, Sonali [1 ]
Pradhan, Sukanta Kumar [2 ]
Ray, Pratima [1 ]
机构
[1] Orissa Univ Agr & Technol, CBSH, Dept Microbiol, Unit 7, Bhubaneswar 751003, Orissa, India
[2] Orissa Univ Agr & Technol, Ctr Post Grad Studies, Dept Bioinformat, Bhubaneswar 751003, Orissa, India
关键词
POTENTIAL APPLICATION; ENZYMES; FERMENTATION; ALKALIPHILES; OPTIMIZATION; PROTEASES; BACTERIA; LIPASE;
D O I
10.1016/j.ijbiomac.2019.11.149
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An alkaliphile bacterial strain designated as CH11 was isolated from the sediments of Chilika Lake, Odisha. The isolate showed stupendous growth and production of alpha-amylase at pH 10.0. Through 16S rRNA gene based molecular technique this isolate was identified as Bacillus cereus strain SP-CH11 having GenBank Accession No. KT992791. Homogenous similar to 55 kDa extracellular alpha-amylase was extracted with 241.304, 26.26 and 3.2-fold acceleration in specific activity, purification fold and yield respectively. The alkaline alpha-amylase AA11 was further characterized. At pH 9.0 the purified enzyme AA11 was highly stable while retaining 88-100% functional viability at temperature range from 35 to 65 degrees C, confirming its thermostability nature. It showed stability with powdered and liquid detergents at 7 mg/mL and 100-fold dilutions respectively. AA11 efficiently removed the starch stain from cotton fabrics. The findings of this study indicate that the isolate CH11 is a source of novel alkaline alpha-amylase that has promising application in food and detergent industries. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:804 / 812
页数:9
相关论文
共 48 条
[1]   Extraction, Purification and Characterization of Thermostable, Alkaline Tolerant α-Amylase from Bacillus cereus [J].
Annamalai, N. ;
Thavasi, R. ;
Vijayalakshmi, S. ;
Balasubramanian, T. .
INDIAN JOURNAL OF MICROBIOLOGY, 2011, 51 (04) :424-429
[2]  
[Anonymous], ADV APPL MICROBIOLOG
[3]  
[Anonymous], ASIAN J MICROBIOL BI
[4]  
[Anonymous], INT J BIOL MACROMOL
[5]  
Aygan A, 2008, BRAZ J MICROBIOL, V39, P547, DOI 10.1590/S1517-838220080003000027
[6]   Isolation and characterization of a novel thermostable α-amylase from Korean pine seeds [J].
Azad, Md Abul Kalam ;
Bae, Jae-Han ;
Kim, Jong-Sang ;
Lim, Jin-Kyu ;
Song, Kyung-Sik ;
Shin, Beom-Soo ;
Kim, Hak-Ryul .
NEW BIOTECHNOLOGY, 2009, 26 (3-4) :143-149
[7]   Production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by Bacillus spp. [J].
Bhagowati, Pabitra ;
Pradhan, Shreema ;
Dash, Hirak R. ;
Das, Surajit .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2015, 79 (09) :1454-1463
[8]   APPLICATION OF MICROBIAL α-AMYLASE IN INDUSTRY - A REVIEW [J].
de Souza, Paula Monteiro ;
de Oliveira e Magalhaes, Perola .
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2010, 41 (04) :850-861
[9]   Production and partial characterization of extracellular amylase enzyme from Bacillus amyloliquefaciens P-001 [J].
Deb, Promita ;
Talukdar, Saimon Ahmad ;
Mohsina, Kaniz ;
Sarker, Palash Kumar ;
Abu Sayem, S. M. .
SPRINGERPLUS, 2013, 2 :1-12
[10]   Cold-tolerant endoglucanase producing ability of Mrakia robertii A2-3 isolated from cryoconites, Hamtha glacier, Himalaya [J].
Dhume, Gandhali M. ;
Maharana, Abhas K. ;
Tsuji, Masaharu ;
Srivastava, Alok K. ;
Singh, Shiv M. .
JOURNAL OF BASIC MICROBIOLOGY, 2019, 59 (07) :667-679