Combinatorial modification of degradable polymers enables transfection of human cells comparable to adenovirus

被引：138
|
作者：
Green, Jordan J.
Zugates, Gregory T.
Tedford, Nathan C.
Huang, Yu-Hung
Griffith, Linda G.
Lauffenburger, Douglas A.
Sawicki, Janet A.
Langer, Robert
Anderson, Daniel G.
机构：
[1] MIT, Canc Res Ctr, Cambridge, MA 02139 USA
[2] MIT, Div Biol Engn, Cambridge, MA 02139 USA
[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[4] Lankenau Inst Med Res, Wynnewood, PA 19096 USA
来源：
ADVANCED MATERIALS | 2007年 / 19卷 / 19期
关键词：
D O I：
10.1002/adma.200700371
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
End-modified poly(beta-amino ester)s, easy-to-synthesize degradable polymers, are able to deliver DNA to primary human cells at levels comparable to adenovirus and two orders of magnitude better than the commonly used non-viral vector, polyethylenimine. Small structural changes are found to affect multiple steps of gene delivery including the DNA binding affinity, nanoparticle size, intracellular DNA uptake, and final protein expression. In vivo, these polymer modifications enhance DNA delivery to ovarian tumors.
引用
收藏
页码：2836 / +
页数：8
相关论文
共 44 条
  • [1] Chitosan Modification of Adenovirus to Modify Transfection Efficiency in Bovine Corneal Epithelial Cells
    Wang, I-Jong
    Jhuang, Min-Chao
    Chen, Yu-Hsin
    Yeh, Lung-Kun
    Liu, Chia-Yang
    Young, Tai-Horng
    PLOS ONE, 2010, 5 (08):
  • [2] Hexon modification of human adenovirus type 5 vectors enables efficient transduction of human multipotent mesenchymal stromal cells
    Nilson, Robin
    Luebbers, Olivia
    Schmidt, Christoph Q.
    Rojewski, Markus
    Zeplin, Philip Helge
    Funk, Wolfgang
    Schrezenmeier, Hubert
    Kritzinger, Astrid
    Kochanek, Stefan
    Krutzke, Lea
    MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT, 2022, 25 : 96 - 110
  • [3] END-JOINING OF DNA FRAGMENTS IN ADENOVIRUS TRANSFECTION OF HUMAN-CELLS
    MUNZ, PL
    YOUNG, CSH
    VIROLOGY, 1991, 183 (01) : 160 - 169
  • [4] MODIFICATION OF RADIATION DRUG SENSITIVITY IN CHO CELLS BY TRANSFECTION WITH HUMAN DNA
    SAKAI, K
    JOURNAL OF RADIATION RESEARCH, 1987, 28 (01) : 92 - 92
  • [5] Efficient gene delivery into human dendritic cells by adenovirus polyethylenimine and mannose polyethylenimine transfection
    Diebold, SS
    Lehrmann, H
    Kursa, M
    Wagner, E
    Cotten, M
    Zenke, M
    HUMAN GENE THERAPY, 1999, 10 (05) : 775 - 786
  • [6] IMMORTALIZATION OF HUMAN PROXIMAL TUBULE CELLS BY TRANSFECTION OF HUMAN ADENOVIRUS 5 E1A GENE
    Ying, Xuming
    Wang, Xiaoyun
    NEPHROLOGY, 2005, 10 : A124 - A124
  • [7] Pervasive combinatorial modification of histone H3 in human cells
    Garcia, Benjamin A.
    Pesavento, James J.
    Mizzen, Craig A.
    Kelleher, Neil L.
    NATURE METHODS, 2007, 4 (06) : 487 - 489
  • [8] Pervasive combinatorial modification of histone H3 in human cells
    Benjamin A Garcia
    James J Pesavento
    Craig A Mizzen
    Neil L Kelleher
    Nature Methods, 2007, 4 : 487 - 489
  • [9] Nonviral and viral gene transfer into different subsets of human dendritic cells yield comparable efficiency of transfection
    Lundqvist, A
    Noffz, G
    Pavlenko, M
    Sæboe-Larssen, S
    Fong, T
    Maitland, N
    Pisa, P
    JOURNAL OF IMMUNOTHERAPY, 2002, 25 (06) : 445 - 454
  • [10] Transfection of human lactotroph adenoma cells with an adenovirus vector expressing tyrosine hydroxylase decreases prolactin release
    Freese, A
    During, MJ
    Davidson, BL
    Gennarelli, TA
    Kaplitt, MG
    Flamm, ES
    Snyder, PJ
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1996, 81 (06): : 2401 - 2404
12345