Graphitic carbon nitride quantum dots as analytical probe for viewing sialic acid on the surface of cells and tissues

被引：28

作者：

Zhang, Mo ^{[1
,2
]}

Wang, Qing ^{[1
,2
]}

Xu, Yupin ^{[1
,2
]}

Guo, Lei ^{[1
,2
]}

Lai, Zhizhen ^{[1
,2
]}

Li, Zhili ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Inst Basic Med Sci, Dept Biophys & Struct Biol, Beijing 100005, Peoples R China

[2] Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2020年 / 1095卷

基金：

中国国家自然科学基金;

关键词：

Sialic acids; Carbon nitride; Quantum dots; Living cells; Fluorescent probe; PHENYLBORONIC ACID; HYDROGEN EVOLUTION; CANCER; BINDING; GLYCOSYLATION; GLYCOPROTEINS; FABRICATION; NANOSHEETS; BIOLOGY; FACILE;

D O I：

10.1016/j.aca.2019.10.031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The abnormal expression of sialic acids (SAs) on cells and tissues is closely related to various pathophysiological states. Here we applied phenylboronic acid (PBA) functionalized graphitic carbon nitride fluorescent quantum dots (PCQDs) with sizes from 3 to 5 nm in efficient and selective labeling SAs on the surface of living cells and tissues. With abundant PBA in their structure, the water soluble PCQDs showed the relative SA level on the cell surface via selectively and efficiently staining different cell lines in 30 min and revealed that M1 macrophages may express more SAs on their surfaces compared with M0 and M2. The distinct demarcation of cancerous and para-noncancerous areas on cancer tissue sections was showed by PCQDs staining. PCQDs with their high selectivity, stable photoluminescence, low cost, and nontoxicity can be an ideal SA fluorescent probe for living cells and tissues. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：204 / 211

页数：8

共 55 条

[31] Cryptic sialic acid binding lectins on human blood leukocytes can be unmasked by sialidase treatment or cellular activation [J].