Atrazine induces transcriptional changes in marker genes associated with steroidogenesis in primary cultures of rat Leydig cells

Reproductive toxicity of atrazine (ATZ) is well reported in mammals. However, the underlying mechanisms are poorly understood and need to be explored. Thus, we investigate ATZ induced transcriptional changes in selected markers of steroidogenesis in primary cultures of rat interstitial Leydig cells (ILCs). Cytotoxic studies were carried out by exposing the cells to ATZ (0.5-50 mu g/mL or 2.32-232 mu M) for 24-72 h, whereas; the exposure period of expression studies was for 2 h. ATZ exposure of (25 and 50 mu g/ml) for 48 h onwards was found to be cytotoxic in MTT (dimethylthiazol-diphenyl tetrazolium bromide salt) assay, while in NRU (neutral red uptake) assay, cytotoxicity could be recorded at 50 mu g/ml exposure of 72 h only. A significant dose dependent induction in the levels of mRNA expression of genes of steroidogenic acute regulatory protein (STAR), cytochrome P45011A1, 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), and other steroidogenic proteins were observed in cells exposed to ATZ. Our data suggest the applicability of these selected marker genes of steroidogenesis as an indicator of short term exposure of ATZ induced testicular toxicity in rats interstitial Leydig cells (ILCs). (C) 2011 Elsevier Ltd. All rights reserved.