Chitinase system ofAeromonas salmonicida, and characterization of enzymes involved in chitin degradation

被引：24

作者：

Pentekhina, Iuliia ^{[1
,2
]}

Hattori, Tatsuyuki ^{[1
]}

Dinh Minh Tran ^{[3
]}

Shima, Mizuki ^{[4
]}

Watanabe, Takeshi ^{[1
,4
,6
]}

Sugimoto, Hayuki ^{[1
,4
]}

Suzuki, Kazushi ^{[1
,4
,5
]}

机构：

[1] Niigata Univ, Grad Sch Sci & Technol, Niigata, Japan

[2] Far Eastern Fed Univ, Sch Econ & Management, Vladivostok, Russia

[3] Tay Nguyen Univ, Inst Biotechnol & Environm, Buon Ma Thuot, Vietnam

[4] Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata, Japan

[5] Niigata Univ, Sakeol Ctr, Niigata, Japan

[6] Niigata Agrofood Univ, Fac Agrofood Sci, Niigata, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2020年 / 84卷 / 09期

关键词：

Aeromonas salmonicida; chitinase; lytic polysaccharide monooxygenase; synergistic effect; antifungal activity; FAMILY; 19; CHITINASE; BINDING DOMAIN; LOOP STRUCTURE; CLONING; IDENTIFICATION; EXPRESSION; BACTERIUM; SEQUENCE; STRAIN; GENE;

D O I：

10.1080/09168451.2020.1771539

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genes encoding chitin-degrading enzymes inAeromonas salmonicidaSWSY-1.411 were identified and cloned inEscherichia coli. The strain contained two glycoside hydrolase (GH) families 18 chitinases: AsChiA and AsChiB, two GH19 chitinases: AsChiC and AsChiD, and an auxiliary activities family 10 protein, lytic polysaccharide monooxygenase: AsLPMO10A. These enzymes were successfully expressed inE. coliand purified. AsChiB had the highest hydrolytic activity against insoluble chitin. AsChiD had the highest activity against water-soluble chitin. The peroxygenase activity of AsLPMO10A was lower compared to SmLPMO10A fromSerratia marcescens. Synergism on powdered chitin degradation was observed when AsChiA and AsLPMO10A were combined with other chitinases of this strain. More than twice the increase of the synergistic effect was observed when powdered chitin was treated by a combination of AsLPMO10A with all chitinases. GH19 chitinases suppressed the hyphal growth ofTrichoderma reesei.

引用

页码：1936 / 1947

页数：12

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