S-(-)-nicotine-1'-N-oxide reductase activity of rat liver aldehyde oxidase

被引：0

作者：

Sugihara, K

Kitamura, S

Tatsumi, K

机构：

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1996年 / 40卷 / 03期

关键词：

S-(-)-nicotine-1'-N-oxide; S-(-)-nicotine; N-oxide reduction; rat liver cytosol; aldehyde oxidase;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, a rat liver cytosolic enzyme responsible for reduction of S-(-)-nicotine-1'-N-oxide to S-(-)-nicotine was investigated. We found that aldehyde oxidase (EC 1.2.3.1) can function as a S-(-)-nicotine-1'-N-oxide reductase in the presence of its electron donor such as 2-hydroxypyrimidine. The apparent Km and Vmax values of the enzyme for the N-oxide were 0.24 mM and 30.3 nmol/10 min/mg protein, respectively.

引用

页码：535 / 541

页数：7

共 14 条

[1] MOLYBDENUM HYDROXYLASES AS DRUG-METABOLIZING-ENZYMES [J].