AbeM, an H+-coupled Acinetobacter baumannii multidrug efflux pump belonging to the MATE family of transporters

被引:156
作者
Su, XZ [1 ]
Chen, J [1 ]
Mizushima, T [1 ]
Kuroda, T [1 ]
Tsuchiya, T [1 ]
机构
[1] Okayama Univ, Dept Microbiol, Fac Pharmaceut Sci, Okayama 7008530, Japan
关键词
D O I
10.1128/AAC.49.10.4362-4364.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We cloned a gene, abeM, for a multidrug efflux pump from Acinetobacter baumannii using Escherichia coli as the host. Sequence analysis revealed that AbeM is a member of the MATE family of pumps. AbeM was found to be an H+-coupled multidrug efflux pump and a unique member of the MATE family.
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页码:4362 / 4364
页数:3
相关论文
共 24 条
[1]   Characterization of OXA-25, OXA-26, and OXA-27, molecular class D β-lactamases associated with carbapenem resistance in clinical isolates of Acinetobacter baumannii [J].
Afzal-Shah, M ;
Woodford, N ;
Livermore, DM .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (02) :583-588
[2]   Unique features revealed by the genome sequence of Acinetobacter sp ADP1, a versatile and naturally transformation competent bacterium [J].
Barbe, V ;
Vallenet, D ;
Fonknechten, N ;
Kreimeyer, A ;
Oztas, S ;
Labarre, L ;
Cruveiller, S ;
Robert, C ;
Duprat, S ;
Wincker, P ;
Ornston, LN ;
Weissenbach, J ;
Marlière, P ;
Cohen, GN ;
Médigue, C .
NUCLEIC ACIDS RESEARCH, 2004, 32 (19) :5766-5779
[3]   Acinetobacter spp, as nosocomial pathogens: Microbiological, clinical, and epidemiological features [J].
BergogneBerezin, E ;
Towner, KJ .
CLINICAL MICROBIOLOGY REVIEWS, 1996, 9 (02) :148-+
[4]   The multidrug efflux protein NorM is a prototype of a new family of transporters [J].
Brown, MH ;
Paulsen, IT ;
Skurray, RA .
MOLECULAR MICROBIOLOGY, 1999, 31 (01) :394-395
[5]   VmrA, a member of a novel class of Na+-coupled multidrug efflux pumps from Vibrio parahaemolyticus [J].
Chen, J ;
Morita, Y ;
Huda, MN ;
Kuroda, T ;
Mizushima, T ;
Tsuchiya, T .
JOURNAL OF BACTERIOLOGY, 2002, 184 (02) :572-576
[6]   Imipenem resistance among Acinetobacter baumannii: Association with reduced expression of a 33-36 kDa outer membrane protein [J].
Clark, RB .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1996, 38 (02) :245-251
[7]   Relationship between β-lactamase production, outer membrane protein and penicillin-binding protein profiles on the activity of carbapenems against clinical isolates of Acinetobacter baumannii [J].
Fernández-Cuenca, F ;
Martínez-Martínez, L ;
Conejo, MC ;
Ayala, JA ;
Perea, EJ ;
Pascual, A .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 51 (03) :565-574
[8]   An H+-coupled multidrug efflux pump, PmpM, a member of the MATE family of transporters, from Pseudomonas aeruginosa [J].
He, GX ;
Kuroda, T ;
Mima, T ;
Morita, Y ;
Mizushima, T ;
Tsuchiya, T .
JOURNAL OF BACTERIOLOGY, 2004, 186 (01) :262-265
[9]   Selection of topoisomerase mutations and overexpression of adeB mRNA transcripts during an outbreak of Acinetobacter baumannii [J].
Higgins, PG ;
Wisplinghoff, H ;
Stefanik, D ;
Seifert, H .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2004, 54 (04) :821-823
[10]   Gene cloning and characterization of VcrM, a Na+-coupled multidrug efflux pump, from Vibrio cholerae non-O1 [J].
Huda, MN ;
Chen, J ;
Morita, Y ;
Kuroda, T ;
Mizushima, T ;
Tsuchiya, T .
MICROBIOLOGY AND IMMUNOLOGY, 2003, 47 (06) :419-427