Metabolic responses during hemodialysis determined by quantitative 1H NMR spectroscopy

被引：7

作者：

Fujiwara, Masako ^{[1
]}

Ando, Itiro ^{[2
]}

Takeuchi, Kazuhisa ^{[1
,3
]}

Oguma, Shiro ^{[4
]}

Sato, Hiroshi ^{[2
]}

Sekino, Hiroshi ^{[3
]}

Sato, Keisuke ^{[4
]}

Imai, Yutaka ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Dept Planning Drug Dev & Clin Evaluat, Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, Grad Sch Pharmaceut Sci, Lab Clin Pharmacol & Therapeut, Aoba Ku, Sendai, Miyagi 9808578, Japan

[3] Koujinkai Cent Clin, Miyagino Ku, Sendai, Miyagi 9830852, Japan

[4] Koujinkai Nagamachi Clin, Taihaku Ku, Sendai, Miyagi 9820001, Japan

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2015年 / 111卷

关键词：

Hemodialysis; Lactate; Metabolic response; H-1 NMR spectroscopy; Pyruvate; AMINO-ACID;

D O I：

10.1016/j.jpba.2015.03.035

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A large proportion of patients with end-stage renal disease have lifelong hemodialysis (HD) treatment. HD rapidly and indiscriminately removes necessary small metabolites together with uremic toxins from plasma into dialysate. To investigate metabolic responses to HD, we determined the levels of metabolites through time-course monitoring of 'H NMR spectroscopy of dialysate during HD. The dialysate sample is stable for analysis because it contains only small metabolites without proteins. It was collected non-invasively from 9 HD patients with chronic glomerular nephropathy, at 6 time points during 4h of HD in 5 sessions. Creatinine, alanine, lactate, pyruvate and valine were simultaneously quantified on a one-dimensional single-pulse spectrum with a single standard compound. The concentration of creatinine exhibited monotonous decay with time, while that of valine decreased slowly and then maintained its levels throughout an HD. lactate, alanine and pyruvate increased at 2-3 h after the initiation of HD. They exhibited remarkable responses to HD with production from the body. The time-course of change in the 4 metabolites of lactate, pyruvate, alanine, and valine had reproducible behavior unique to each patient during the HD. This finding may be applied to distinguish metabolic status in HD patients. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：159 / 162

页数：4

共 8 条

[1] 1H NMR Spectroscopic Quantification of Plasma Metabolites in Dialysate during Hemodialysis [J].