EIGER detector: application in macromolecular crystallography

被引：101

作者：

Casanas, Arnau ^{[1
]}

Warshamanage, Rangana ^{[1
]}

Finke, Aaron D. ^{[1
]}

Panepucci, Ezequiel ^{[1
]}

Olieric, Vincent ^{[1
]}

Noell, Anne ^{[2
]}

Tampe, Robert ^{[2
]}

Brandstetter, Stefan ^{[3
]}

Foerster, Andreas ^{[3
]}

Mueller, Marcus ^{[3
]}

Schulze-Briese, Clemens ^{[3
]}

Bunk, Oliver ^{[1
]}

Wang, Meitian ^{[1
]}

机构：

[1] Paul Scherrer Inst, Swiss Light Source, CH-5232 Villigen, Switzerland

[2] Goethe Univ Frankfurt, Inst Biochem, Bioctr, Max von Laue Str 9, D-60438 Frankfurt, Germany

[3] DECTRIS Ltd, Taefernweg 1, CH-5405 Baden, Switzerland

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2016年 / 72卷

关键词：

X-ray detectors; EIGER detector; macromolecular crystallography; data-collection strategy; DATA-COLLECTION STRATEGY; X-RAY CRYSTALLOGRAPHY; PROTEIN CRYSTALLOGRAPHY; SERIAL CRYSTALLOGRAPHY; RADIATION-DAMAGE; NEXT-GENERATION; INTEGRATION; REFINEMENT; REDUCTION;

D O I：

10.1107/S2059798316012304

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 x 75 mu m, frame rates of up to 3000 Hz and a dead time as low as 3.8 mu s. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine phi-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.

引用

页码：1036 / 1048

页数：13

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