Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

被引：32

作者：

da Silva, Luiz Claudio ^{[1
]}

Garcia, Thiago ^{[1
]}

Mori, Michela ^{[2
]}

Sandri, Giuseppina ^{[2
]}

Bonferoni, Maria Cristina ^{[2
]}

Finotelli, Priscila V. ^{[1
]}

Cinelli, Leonardo P. ^{[1
]}

Caramella, Carla ^{[2
]}

Cabral, Lucio M. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Drug Dept, BR-21941902 Rio De Janeiro, RJ, Brazil

[2] Univ Pavia, Dept Drug Sci, I-27100 Pavia, Italy

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2012年 / 7卷

关键词：

nanoparticles; chitosan; chondroitin sulfate; fucoidan; aPTT; Caco-2; CHITOSAN-CHONDROITIN SULFATE; DRUG-DELIVERY; CAPILLARY-ELECTROPHORESIS; CONTROLLED-RELEASE; SYSTEMS; COMPLEX; FUCAN; DOXORUBICIN; ALGINATE; CARRIERS;

D O I：

10.2147/IJN.S31632

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.

引用

页码：2975 / 2986

页数：12

共 51 条

[1]

Asimakopoulou AP, 2008, IN VIVO, V22, P385

[2] Biochemical basis of the pharmacologic action of chondroitin sulfates on the osteoarticular system [J].

Bali, JP ;

Cousse, H ;

Neuzil, E .

SEMINARS IN ARTHRITIS AND RHEUMATISM, 2001, 31 (01) :58-68

[3]

BARKALOW FJ, 1994, J BIOL CHEM, V269, P3957

[4] Conformation of sulfated galactan and sulfated fucan in aqueous solutions: Implications to their anticoagulant activities [J].

Becker, Camila F. ;

Guimaraes, Jorge A. ;

Mourao, Paulo A. S. ;

Verli, Hugo .

JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 2007, 26 (01) :391-399

[5] Layer-by-layer assembly of polysaccharide-based nanostructured surfaces containing polyelectrolyte complex nanoparticles [J].

Boddohi, Soheil ;

Almodovar, Jorge ;

Zhang, Hao ;

Johnson, Patrick A. ;

Kipper, Matti J. .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2010, 77 (01) :60-68

[6] Chitosan and chitosan ethylene oxide propylene oxide block copolymer nanoparticles as novel carriers for proteins and vaccines [J].

Calvo, P ;

RemunanLopez, C ;

VilaJato, JL ;

Alonso, MJ .

PHARMACEUTICAL RESEARCH, 1997, 14 (10) :1431-1436

[7] Endocytosis at the nanoscale [J].

Canton, Irene ;

Battaglia, Giuseppe .

CHEMICAL SOCIETY REVIEWS, 2012, 41 (07) :2718-2739

[8] The characteristics, biodistribution and bioavailability of a chitosan-based nanoparticulate system for the oral delivery of heparin [J].

Chen, Mei-Chin ;

Wong, Hen-Sheng ;

Lin, Kun-Ju ;

Chen, Hsin-Lung ;

Wey, Shiaw-Pyng ;

Sonaje, Kiran ;

Lin, Yu-Hsin ;

Chu, Che-Yi ;

Sung, Hsing-Wen .

BIOMATERIALS, 2009, 30 (34) :6629-6637

[9] Biological activities of sulfated polysaccharides from tropical seaweeds [J].

Costa, L. S. ;

Fidelis, G. P. ;

Cordeiro, S. L. ;

Oliveira, R. M. ;

Sabry, D. A. ;

Camara, R. B. G. ;

Nobre, L. T. D. B. ;

Costa, M. S. S. P. ;

Almeida-Lima, J. ;

Farias, E. H. C. ;

Leite, E. L. ;

Rocha, H. A. O. .

BIOMEDICINE & PHARMACOTHERAPY, 2010, 64 (01) :21-28

[10] Comparison of the anticoagulant action of sulfated and phosphorylated polysaccharides [J].

Dace, R ;

McBride, E ;

Brooks, K ;

Gander, J ;

Buszko, M ;

Doctor, VM .

THROMBOSIS RESEARCH, 1997, 87 (01) :113-121

← 1 2 3 4 5 6 →