Prefractionation of cerebrospinal fluid to enhance glycoprotein concentration prior to structural determination with FT-ICR mass spectrometry

被引：15

作者：

Sihlbom, C

Davidsson, P

Nilsson, CL

机构：

[1] Univ Gothenburg, Inst Med Biochem, SE-40530 Gothenburg, Sweden

[2] AstraZeneca R&D Molndal, Discovery Med Mol Pharmacol, SE-43183 Molndal, Sweden

[3] Natl High Magnet Field Lab, Tallahassee, FL 32310 USA

[4] Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 06期

关键词：

glycoprotein; prefractionation; CSF; 2-DE; albumin removal; FT-ICR MS;

D O I：

10.1021/pr050210g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycoproteins in cerebrospinal fluid are found to be altered in Alzheimer patients compared to healthy control individuals. We have utilized micro-solution isoelectric focusing and affinity chromatography, prior to gel electrophoresis to enable site-specific structural determination of the N-linked glycans in apolipoprotein J with the use of FT-ICR MS. The albumin depletion method is the most suitable as prefractionation method of CSF prior to 2-DE for structural determination of glycoproteins in the study of neurodegenerative disorders.

引用

收藏

页码：2294 / 2301

页数：8

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