Novel Phage Amplified Multichannel Series Piezoelectric Quartz Crystal Sensor for Rapid and Sensitive Detection of Mycobacterium tuberculosis

被引:41
作者
Mi, Xianwen [1 ,2 ]
He, Fengjiao [1 ]
Xiang, Meiyu [1 ]
Lian, Yan [1 ]
Yi, Songlin [3 ]
机构
[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China
[2] Huaihua Med Coll, Huaihua 418000, Hunan, Peoples R China
[3] Hunan Inst TB Control, Changsha 410006, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
BACTERIOPHAGE-BASED METHOD; MGIT; 960; SYSTEM; DIAGNOSIS; SMEGMATIS; GROWTH; SUSCEPTIBILITY; ASSAY; IRON;
D O I
10.1021/ac2020728
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The key factors that control the spread and mortality rate of tuberculosis (TB) are rapid detection and diagnosis. However, the current detection of Mycobacterium tuberculosis (M. tuberculosis) cannot meet the recommended requirements for clinical diagnosis in turnaround time. In this paper, the feature of phage D29 that infects M. tuberculosis and Mycobacterium smegmatis (M. smegmatis) was combined with the sensitivity of multichannel series piezoelectric quartz crystal sensor (MSPQC) to detect M. tuberculosis. The phage D29 played a role of inhibiting the growth of M. tuberculosis and M. smegmatis. M. tuberculosis is used to protect phage D29 from being killed by ferrous ammonium sulfate (FAS) and carries phage D29 into the detection medium containing M. smegmatis. The action of M. smegmatis indicated the existence state of phage D29 in the detection medium. The growth curve of M. smegmatis obtained by MSPQC indicated the state of the growth of M. tuberculosis. Therefore, M. tuberculosis in the sample could be rapidly detected by evaluating the extent of inhibiting the growth of M. smegmatis compared with the normal growth of M. smegmatis. The detection of M. tuberculosis was transformed into the detection of M. smegmatis, which is more rapid and sensitive than that of M. tuberculosis. For 102 cfu/mL of M. tuberculosis in clinical sample, the turnaround time was less than 30 h. Although statistical analysis showed that no significant difference existed between the results of the proposed method here and the BACTEC960 MGIT method in clinical M. tuberculosis detection, the phage amplified MSPQC (PA MSPQC) method presented here was faster and more economical.
引用
收藏
页码:939 / 946
页数:8
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