The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma

被引：25
作者：
Hagenaars, M
Koelemij, R
Ensink, NG
van Eendenburg, JDH
van Vlierberghe, RLP
Eggermont, AMM
van de Velde, CJH
Fleuren, GJ
Kuppen, PJK
机构：
[1] Leiden Univ, Med Ctr, Dept Surg, NL-2300 RC Leiden, Netherlands
[2] Leiden Univ, Med Ctr, Dept Pathol, NL-2300 RC Leiden, Netherlands
[3] Univ Rotterdam Hosp, Daniel den Hoed Canc Ctr, Dept Surg, Rotterdam, Netherlands
来源：
CLINICAL & EXPERIMENTAL METASTASIS | 2000年 / 18卷 / 04期
关键词：
colon tumour; monoclonal antibody; rat;
D O I：
10.1023/A:1011062002851
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.
引用
收藏
页码：281 / 289
页数：9
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