Effect of lithium chloride on cell proliferation and osteogenic differentiation in stem cells from human exfoliated deciduous teeth

被引：22
作者：
Rattanawarawipa, Panarat [1 ,2 ]
Pavasant, Prasit [2 ,3 ]
Osathanon, Thanaphum [2 ,3 ]
Sukarawan, Waleerat [1 ,2 ]
机构：
[1] Chulalongkorn Univ, Dept Pediat Dent, Fac Dent, Bangkok 10330, Thailand
[2] Chulalongkorn Univ, Mineralized Tissue Res Unit, Fac Dent, Bangkok 10330, Thailand
[3] Chulalongkorn Univ, Dept Anat, Fac Dent, Bangkok 10330, Thailand
来源：
TISSUE & CELL | 2016年 / 48卷 / 05期
关键词：
Lithium chloride; Stem cells; Proliferation; Osteogenic; Differentiation; WNT SIGNALING PATHWAY; GSK3; INHIBITORS; CYCLE ARREST; ACTIVATION; MECHANISM; BETA;
D O I：
10.1016/j.tice.2016.08.005
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Lithium Chloride (LiCl) has been used as a canonical Wnt pathway activator due to its ability to inhibit a glycogen synthase kinase-3. The aim of the present study was to investigate the effect of LiCl on cell proliferation and osteogenic differentiation in stem cells isolated from human exfoliated deciduous teeth (SHEDs). SHEDs were isolated and cultured in media supplemented with LiCl at 5,10, or 20 mM. The results demonstrated that LiCl significantly decreased SHEDs colony forming unit ability in a dose dependent manner. LiCl significantly enhanced the percentage of cells in the sub GO phase, accompanied by a reduction of the percentage of cells in the G1 phase at day 3 and 7 after treatment. Further, LiCl markedly decreased OSX and DMP1 mRNA expression after treating SHEDs in an osteogenic induction medium for 7 days. In addition, no significant difference in alkaline phosphatase enzymatic activity or mineral deposition was found. Together, these results imply that LiCl influences SHEDs behavior. (C) 2016 Elsevier Ltd. All rights reserved.
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收藏
页码：425 / 431
页数：7
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