Caveolae-dependent Endocytosis Is Required for Class A Macrophage Scavenger Receptor-mediated Apoptosis in Macrophages

被引：102

作者：

Zhu, Xu-Dong ^{[1
,2
]}

Zhuang, Yan ^{[1
]}

Ben, Jing-Jing ^{[1
]}

Qian, Ling-Ling ^{[1
]}

Huang, Han-Peng ^{[1
]}

Bai, Hui ^{[1
]}

Sha, Jia-Hao ^{[2
]}

He, Zhi-Gang ^{[3
]}

Chen, Qi ^{[1
,2
]}

机构：

[1] Nanjing Med Univ, Atherosclerosis Res Ctr, Key Lab Human Funct Genom, Nanjing 210029, Peoples R China

[2] Nanjing Med Univ, Inst Reprod Med, Nanjing 210029, Peoples R China

[3] Harvard Univ, Sch Med, Childrens Hosp, Div Neurosci, Boston, MA 02115 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 10期

基金：

中国国家自然科学基金;

关键词：

LOW-DENSITY-LIPOPROTEIN; FOAM CELL-FORMATION; CYTOPLASMIC DOMAIN; LIPID UPTAKE; PLASMA-MEMBRANE; CLATHRIN; ATHEROSCLEROSIS; INTERNALIZATION; BINDING; CHOLESTEROL;

D O I：

10.1074/jbc.M110.145888

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SR-A (classAmacrophage scavenger receptor) is a transmembrane receptor that can bind many different ligands, including modified lipoproteins that are relevant to the development of vascular diseases. However, the precise endocytic pathways of SR-A/mediated ligands internalization are not fully characterized. In this study, we show that the SR-A/ligand complex can be endocytosed by both clathrin-and caveolae-dependent pathways. Internalizations of SR-A-lipoprotein (such as acLDL) complexes primarily go through clathrin-dependent endocytosis. In contrast, macrophage apoptosis triggered by SR-A-fucoidan internalization requires caveolae-dependent endocytosis. The caveolae-dependent process activates p38 kinase and JNK signaling, whereas the clathrin-mediated endocytosis elicits ERK signaling. Our results suggest that different SR-A endocytic pathways have distinct functional consequences due to the activation of different signaling cascades in macrophages.

引用

页码：8231 / 8239

页数：9

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