Establishment of Pmtoconn Suspension Cultures of Dendrobium candidum for the Production of Bioactive Compounds

被引：11

作者：

Cui, Hai-Yan ^{[1
]}

Murthy, Hosakatte Niranjana ^{[1
,2
]}

Moh, Sang Hyun ^{[3
]}

Cui, Yong-Yi ^{[4
]}

Paek, Kee-Yoeup ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Res Ctr Dev Adv Hort Technol, Cheongju 361763, South Korea

[2] Karnatak Univ, Dept Bot, Dharwad 580003, Karnataka, India

[3] Antiageing Res Inst, Inchon 406840, South Korea

[4] Zheijian Agr & Forestry Univ, Sch Agr & Food Sci, Linan 311300, Hangzjpi, Peoples R China

来源：

HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY | 2015年 / 56卷 / 01期

关键词：

bioactive compounds; Dendrobium; polyphenolics; polysaccharides; protocomis; suspension culture; SHOOT REGENERATION; ADVENTITIOUS ROOTS; MEDIUM PH; GROWTH; PROPAGATION; WITHANOLIDE; BIOMASS; L;

D O I：

10.1007/s13580-015-0082-5

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Dendrobium canclidum is an important medicinal plant rich in polysaccharides, phenols, flavonoids and its bioactive compounds are known for various pharmacological activities. Natural populations of D. candidum are depleting due to overexploitation and increasing demand for medicinal and health products. An efficient protocol for protocomi suspension cultures of Dendrobium candidum in shake flasks was developed and the effect of auxins, basal media, nitrogen concentration, sucrose concentration, organic supplements like banana homogenate, coconut water and potato extract vere investigated for the production of biomass and bioactive compounds. For the production of polysaccharides, polyphenolics, and flavonoids, the optimized culture conditions were found to be MS basal medium supplemented with 0.5 mg.L-1 NAA, 5:25 mM NH4-NO3 as nitrogen source, 2.5% (w/v) sucrose, and 1% (v/v) banana homogenate. Results of the current study could be used for scale-up process.

引用

页码：114 / 122

页数：9

共 38 条

[1]

Arditti J., 1993, MICROPORPAGATION ORC, P609

[2]

Bao X. S., 2001, MED PLANTS DENDROBIU

[3] Medium salt strength induced changes in growth, physiology and secondary metabolite content in adventitious roots of Morinda citrifolia: the role of antioxidant enzymes and phenylalanine ammonia lyase [J].

Baque, Md. Abdullahil ;

Lee, Eun-Jung ;

Paek, Kee-Yoeup .

PLANT CELL REPORTS, 2010, 29 (07) :685-694

[4]

Chu CC., 1978, PROC S PLANT TISSUE, P43

[5] Production of biomass and bioactive compounds in protocorm cultures of Dendrobium candidum Wall ex Lindl. using balloon type bubble bioreactors [J].

Cui, Hai-Yan ;

Murthy, Hosakatte Niranjana ;

Moh, Sang Hyun ;

Cui, Yong-Yi ;

Lee, Eun-Jung ;

Paek, Kee-Yoeup .

INDUSTRIAL CROPS AND PRODUCTS, 2014, 53 :28-33

[6] Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor [J].

Cui, Xi-Hua ;

Murthy, Hosakatte Niranjana ;

Jin, You-Xun ;

Yim, Yong-Hyeon ;

Kim, Ji-Yeong ;

Paek, Kee-Yoeup .

BIORESOURCE TECHNOLOGY, 2011, 102 (21) :10072-10079

[7]

Fujita Y, 1981, Plant Cell Rep, V1, P59, DOI 10.1007/BF00269272

[8] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].

GAMBORG, OL ;

MILLER, RA ;

OJIMA, K .

EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+

[9] Micropropagation of Cymbidium sinense using continuous and temporary airlift bioreactor systems [J].

Gao, Ri ;

Wu, Song-Quan ;

Piao, Xuan-Chun ;

Park, So-Young ;

Lian, Mei-Lan .

ACTA PHYSIOLOGIAE PLANTARUM, 2014, 36 (01) :117-124

[10] Plant cell cultures for the production of recombinant proteins [J].

Hellwig, S ;

Drossard, J ;

Twyman, RM ;

Fischer, R .

NATURE BIOTECHNOLOGY, 2004, 22 (11) :1415-1422

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