Amphiregulin Supplementation During Pig Oocyte In Vitro Maturation Enhances Subsequent Development of Cloned Embryos by Promoting Cumulus Cell Proliferation

被引：4
作者：
Zhang, Xianjun [1 ,2 ,3 ]
Zhao, Huaxing [1 ,2 ,3 ]
Li, Yanan [1 ,2 ,3 ]
Zhang, Yuxing [1 ,2 ,3 ]
Liang, Yalin [1 ,2 ,3 ]
Shi, Junsong [4 ]
Zhou, Rong [4 ]
Hong, Linjun [1 ,2 ,3 ]
Cai, Gengyuan [1 ,2 ,3 ]
Wu, Zhenfang [1 ,2 ,3 ,5 ,6 ]
Li, Zicong [1 ,2 ,3 ,5 ,6 ]
机构：
[1] South China Agr Univ, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou, Peoples R China
[2] South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou, Peoples R China
[3] South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou, Peoples R China
[4] Guangdong Wens Pig Breeding Technol Co Ltd, Yunfu, Peoples R China
[5] South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou, Peoples R China
[6] South China Agr Univ, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou 510642, Guangdong, Peoples R China
来源：
CELLULAR REPROGRAMMING | 2022年 / 24卷 / 04期
关键词：
IVM; pig; oocytes; cloned embryos; AREG; EPIDERMAL-GROWTH-FACTOR; INDUCED MEIOTIC RESUMPTION; ZINC-FINGER NUCLEASES; INVITRO FERTILIZATION; SIGNALING PATHWAYS; GRANULOSA-CELLS; KNOCKOUT PIGS; FACTOR-ALPHA; EXPANSION; VIVO;
D O I：
10.1089/cell.2022.0015
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The oocyte in vitro maturation (IVM) technique is important in animal husbandry, biomedicine, and human-assisted reproduction. However, the developmental potential of in vitro matured oocytes is usually lower than that of in vivo matured (IVVM) oocytes. Amphiregulin (AREG) is an EGF-like growth factor that plays critical roles in the maturation and development of mammalian oocytes. This study investigated the effects of AREG supplementation during pig oocyte IVM on the subsequent development of cloned embryos. The addition of AREG to pig oocyte IVM medium improved the developmental competence of treated oocyte-derived cloned embryos by enhancing the expansion and proliferation of cumulus cells (CCs) during IVM. The positive effect of AREG on enhancing the quality of IVVM pig oocytes might be due to the activation of proliferation-related pathways in CCs by acting on the AREG receptor. The present study provides an AREG treatment-based method to improve the developmental competence of cloned pig embryos.
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收藏
页码：175 / 185
页数：11
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