Oligosaccharides from agar extends lifespan through activation of unfolded protein response via SIR-2.1 in Caenorhabditis elegans

被引：2

作者：

Desaka, Natsumi ^{[1
]}

Nishikawa, Hitomi ^{[1
]}

Honda, Yuji ^{[1
]}

Matsumoto, Kenji ^{[1
]}

Matsuzaki, Chiaki ^{[2
]}

Mizushima, Katsura ^{[3
]}

Takagi, Tomohisa ^{[3
]}

Naito, Yuji ^{[4
]}

Higashimura, Yasuki ^{[1
]}

机构：

[1] Ishikawa Prefectural Univ, Dept Food Sci, Nonoichi, Ishikawa 9218836, Japan

[2] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa 9218836, Japan

[3] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Mol Gastroenterol & Hepatol, Kyoto 6028566, Japan

[4] Kyoto Prefectural Univ Med, Dept Human Immunol & Nutr Sci, Kyoto 6028566, Japan

来源：

EUROPEAN JOURNAL OF NUTRITION | 2022年 / 61卷 / 08期

基金：

日本学术振兴会;

关键词：

ER stress; Healthspan; Longevity; Nematode; Oligosaccharide; Sir-2; 1; OXIDATIVE STRESS; INFLAMMATION; PATHWAY; GENES; DIET; INDUCTION; DISEASE; DAF-16; COLON;

D O I：

10.1007/s00394-022-02957-1

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Purpose Agaro-oligosaccharides (AGO), hydrolysis products of agarose, is known to have antioxidant and anti-inflammatory properties. Speculating that AGO is effective for preventing aging, we investigated the longevity-supporting effects of AGO and their mechanisms using Caenorhabditis elegans. Methods Caenorhabditis elegans were fed AGO from young adulthood. The lifespan, locomotory activity, lipofuscin accumulation, and heat stress resistance of the worms were examined. To elucidate mechanisms of AGO-mediated longevity, we conducted comprehensive expression analysis using microarrays. Moreover, we used quantitative real-time PCR (qRT-PCR) to verify the genes showing differential expression levels. Furthermore, we measured the lifespan of loss-of-function mutants to determine the genes related to AGO-mediated longevity. Results AGO extended the lifespan of C. elegans, reduced lipofuscin accumulation, and maintained vigorous locomotion. The microarray analysis revealed that the endoplasmic reticulum-unfolded protein response (ER-UPR) and insulin/insulin-like growth factor-1-mediated signaling (IIS) pathway were activated in AGO-fed worms. The qRT-PCR analysis showed that AGO treatment suppressed sir-2.1 expression, which is a negative regulator of ER-UPR. In loss-of-function mutant of sir-2.1, AGO-induced longevity and heat stress resistance were decreased or cancelled completely. Furthermore, the pro-longevity effect of AGO was decreased in loss-of-function mutants of abnormal Dauer formation (daf) -2 and daf-16, which are IIS pathway-related genes. Conclusion AGO delays the C. elegans aging process and extends their lifespan through the activations of ER-UPR and the IIS pathway.

引用

页码：4179 / 4190

页数：12

共 30 条

[21] Activation of the mitochondrial unfolded protein response does not predict longevity in Caenorhabditis elegans [J].

Bennett, Christopher F. ;

Wende, Helen Vander ;

Simko, Marissa ;

Klum, Shannon ;

Barfield, Sarah ;

Choi, Haeri ;

Pineda, Victor V. ;

Kaeberlein, Matt .

NATURE COMMUNICATIONS, 2014, 5

[22] A novel antioxidant peptide purified from defatted round scad (Decapterus maruadsi) protein hydrolysate extends lifespan in Caenorhabditis elegans [J].

Chen, Haiqiang ;

Wang, Shuang ;

Zhou, Aimei ;

Miao, Jianyin ;

Liu, Jingyi ;

Benjakul, Soottawat .

JOURNAL OF FUNCTIONAL FOODS, 2020, 68

[23] Polygonum multiflorum Extract Exerts Antioxidative Effects and Increases Life Span and Stress Resistance in the Model Organism Caenorhabditis elegans via DAF-16 and SIR-2.1 [J].

Saier, Christina ;

Buechter, Christian ;

Koch, Karoline ;

Waetjen, Wim .

PLANTS-BASEL, 2018, 7 (03)

[24] A catechin-enriched green tea extract prevents glucose-induced survival reduction in Caenorhabditis elegans through sir-2.1 and uba-1 dependent hormesis [J].

Deusing, Dorothe Jenni ;

Winter, Sarah ;

Kler, Adolf ;

Kriesl, Erwin ;

Bonnlaender, Bernd ;

Wenzel, Uwe ;

Fitzenberger, Elena .

FITOTERAPIA, 2015, 102 :163-170

[25] Phosphatidylserine modulates response to oxidative stress through hormesis and increases lifespan via DAF-16 in Caenorhabditis elegans [J].

Bo-Kyoung Kim ;

Sang-Kyu Park .

Biogerontology, 2020, 21 :231-244

[26] Phosphatidylserine modulates response to oxidative stress through hormesis and increases lifespan via DAF-16 in Caenorhabditis elegans [J].

Kim, Bo-Kyoung ;

Park, Sang-Kyu .

BIOGERONTOLOGY, 2020, 21 (02) :231-244

[27] The polyphenol quercetin protects the mev-1 mutant of Caenorhabditis elegans from glucose-induced reduction of survival under heat-stress depending on SIR-2.1, DAF-12, and proteasomal activity [J].

Fitzenberger, Elena ;

Deusing, Dorothe J. ;

Marx, Carolin ;

Boll, Michael ;

Lueersen, Kai ;

Wenzel, Uwe .

MOLECULAR NUTRITION & FOOD RESEARCH, 2014, 58 (05) :984-994

[28] Dioscin Activates Endoplasmic Reticulum Unfolded Protein Response for Defense Against Pathogenic Bacteria in Caenorhabditis elegans via IRE-1/XBP-1 Pathway [J].

Xiao, Yi ;

Liu, Fang ;

Wu, Qinyi ;

Zhu, Xinting ;

Yu, Changyan ;

Jiang, Nian ;

Li, Sanhua ;

Liu, Yun .

JOURNAL OF INFECTIOUS DISEASES, 2024, 229 (01) :237-244

[29] The glyoxylate shunt protein ICL-1 protects from mitochondrial superoxide stress through activation of the mitochondrial unfolded protein response [J].

Wang, Guoqiang ;

Laranjeiro, Ricardo ;

Levalley, Stephanie ;

Van Raamsdonk, Jeremy M. ;

Driscoll, Monica .

FREE RADICAL BIOLOGY AND MEDICINE, 2023, 208 :771-779

[30] 3-O-trans-p-coumaroyl-alphitolic acid, a triterpenoid from Zizyphus jujuba, leads to apoptotic cell death in human leukemia cells through reactive oxygen species production and activation of the unfolded protein response [J].

Mitsuhashi, Yohei ;

Furusawa, Yukihiro ;

Aradate, Tadashi ;

Zhao, Qing-Li ;

Moniruzzaman, Rohan ;

Kanamori, Masahiko ;

Noguchi, Kyo ;

Kondo, Takashi .

PLOS ONE, 2017, 12 (08)

← 1 2 3 →