机构:
Med Res Council Lab Mol Biol, Cambridge CB2 0QH, England
Univ Cambridge, Dept Chem, Cambridge CB2 1EW, EnglandMed Res Council Lab Mol Biol, Cambridge CB2 0QH, England
Chin, Jason W.
[1
,2
]
机构:
[1] Med Res Council Lab Mol Biol, Cambridge CB2 0QH, England
[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
AMINO-ACID MUTAGENESIS;
TRANSFER-RNA SYNTHETASE;
PEPTIDYL-TRANSFERASE CENTER;
LIVE MAMMALIAN-CELLS;
ESCHERICHIA-COLI;
PROTEIN FUNCTION;
LIVING CELLS;
SEMISYNTHETIC ORGANISM;
INTRACELLULAR PROTEINS;
ORTHOGONAL TRANSLATION;
D O I:
10.1038/nature24031
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Nature uses a limited, conservative set of amino acids to synthesize proteins. The ability to genetically encode an expanded set of building blocks with new chemical and physical properties is transforming the study, manipulation and evolution of proteins, and is enabling diverse applications, including approaches to probe, image and control protein function, and to precisely engineer therapeutics. Underpinning this transformation are strategies to engineer and rewire translation. Emerging strategies aim to reprogram the genetic code so that noncanonical biopolymers can be synthesized and evolved, and to test the limits of our ability to engineer the translational machinery and systematically recode genomes.
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Amiram, Miriam
;
Haimovich, Adrian D.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Haimovich, Adrian D.
;
Fan, Chenguang
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Fan, Chenguang
;
Wang, Yane-Shih
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Wang, Yane-Shih
;
Aerni, Hans-Rudolf
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Syst Biol Inst, West Haven, CT USA
Yale Univ, Dept Cellular & Mol Physiol, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Aerni, Hans-Rudolf
;
Ntai, Ioanna
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem, Evanston, IL USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Ntai, Ioanna
;
Moonan, Daniel W.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Moonan, Daniel W.
;
Ma, Natalie J.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Ma, Natalie J.
;
Rovner, Alexis J.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Rovner, Alexis J.
;
论文数: 引用数:
h-index:
机构:
Hong, Seok Hoon
;
Kelleher, Neil L.
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem, Evanston, IL USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Kelleher, Neil L.
;
Goodman, Andrew L.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Sch Med, Dept Microbial Pathogenesis, New Haven, CT USA
Yale Univ, Sch Med, Microbial Sci Inst, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Goodman, Andrew L.
;
Jewett, Michael C.
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem & Biol Engn, Evanston, IL USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Jewett, Michael C.
;
Soell, Dieter
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA
Yale Univ, Dept Chem, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Soell, Dieter
;
论文数: 引用数:
h-index:
机构:
Rinehart, Jesse
;
Isaacs, Farren J.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
Boston Coll, Dept Chem, Chestnut Hill, MA 02467 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Chatterjee, Abhishek
;
Lajoie, Marc J.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Lajoie, Marc J.
;
Xiao, Han
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Xiao, Han
;
Church, George M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Church, George M.
;
Schultz, Peter G.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Amiram, Miriam
;
Haimovich, Adrian D.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Haimovich, Adrian D.
;
Fan, Chenguang
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Fan, Chenguang
;
Wang, Yane-Shih
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Wang, Yane-Shih
;
Aerni, Hans-Rudolf
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Syst Biol Inst, West Haven, CT USA
Yale Univ, Dept Cellular & Mol Physiol, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Aerni, Hans-Rudolf
;
Ntai, Ioanna
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem, Evanston, IL USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Ntai, Ioanna
;
Moonan, Daniel W.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Moonan, Daniel W.
;
Ma, Natalie J.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Ma, Natalie J.
;
Rovner, Alexis J.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Rovner, Alexis J.
;
论文数: 引用数:
h-index:
机构:
Hong, Seok Hoon
;
Kelleher, Neil L.
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem, Evanston, IL USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Kelleher, Neil L.
;
Goodman, Andrew L.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Sch Med, Dept Microbial Pathogenesis, New Haven, CT USA
Yale Univ, Sch Med, Microbial Sci Inst, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Goodman, Andrew L.
;
Jewett, Michael C.
论文数: 0引用数: 0
h-index: 0
机构:
Northwestern Univ, Dept Chem & Biol Engn, Evanston, IL USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Jewett, Michael C.
;
Soell, Dieter
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT USA
Yale Univ, Dept Chem, New Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Soell, Dieter
;
论文数: 引用数:
h-index:
机构:
Rinehart, Jesse
;
Isaacs, Farren J.
论文数: 0引用数: 0
h-index: 0
机构:
Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
Yale Univ, Syst Biol Inst, West Haven, CT USAYale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
Boston Coll, Dept Chem, Chestnut Hill, MA 02467 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Chatterjee, Abhishek
;
Lajoie, Marc J.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Lajoie, Marc J.
;
Xiao, Han
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Xiao, Han
;
Church, George M.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Church, George M.
;
Schultz, Peter G.
论文数: 0引用数: 0
h-index: 0
机构:
Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USAScripps Res Inst, Dept Chem, La Jolla, CA 92037 USA