Expanding and reprogramming the genetic code

被引：577

作者：

Chin, Jason W. ^{[1
,2
]}

机构：

[1] Med Res Council Lab Mol Biol, Cambridge CB2 0QH, England

[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

NATURE | 2017年 / 550卷 / 7674期

基金：

英国医学研究理事会;

关键词：

AMINO-ACID MUTAGENESIS; TRANSFER-RNA SYNTHETASE; PEPTIDYL-TRANSFERASE CENTER; LIVE MAMMALIAN-CELLS; ESCHERICHIA-COLI; PROTEIN FUNCTION; LIVING CELLS; SEMISYNTHETIC ORGANISM; INTRACELLULAR PROTEINS; ORTHOGONAL TRANSLATION;

D O I：

10.1038/nature24031

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nature uses a limited, conservative set of amino acids to synthesize proteins. The ability to genetically encode an expanded set of building blocks with new chemical and physical properties is transforming the study, manipulation and evolution of proteins, and is enabling diverse applications, including approaches to probe, image and control protein function, and to precisely engineer therapeutics. Underpinning this transformation are strategies to engineer and rewire translation. Emerging strategies aim to reprogram the genetic code so that noncanonical biopolymers can be synthesized and evolved, and to test the limits of our ability to engineer the translational machinery and systematically recode genomes.

引用

页码：53 / 60

页数：8

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