A new NEDD8-ligating system for cullin-4A

被引：214

作者：

Osaka, F

Kawasaki, H

Aida, N

Saeki, M

Chiba, T

Kawashima, S

Tanaka, K

Kato, S ^{[1
]}

机构：

[1] Japan Sci & Technol Corp, JST, Kato Cytoprot Network Project, ERATO,Sagami Chem Res Ctr, Kanagawa 2290012, Japan

[2] Japan Sci & Technol Corp, CREST, Bunkyo Ku, Tokyo 1130021, Japan

[3] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1130021, Japan

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 15期

关键词：

ubiquitin; ubiquitin-like protein; NEDD8; SUMO-1; cullin-4A; cdc53;

D O I：

10.1101/gad.12.15.2263

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an El (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino-and carboxy-terminal regions of El and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.

引用

页码：2263 / 2268

页数：6

共 31 条

[11] Cell cycle: Cull and destroy [J].

Jackson, PK .

CURRENT BIOLOGY, 1996, 6 (10) :1209-1212

[12] THE UBIQUITIN-CONJUGATION SYSTEM [J].

JENTSCH, S .

ANNUAL REVIEW OF GENETICS, 1992, 26 :179-207

[13] Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p [J].

Johnson, ES ;

Blobel, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (43) :26799-26802

[14] The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer [J].

Johnson, ES ;

Schwienhorst, I ;

Dohmen, RJ ;

Blobel, G .

EMBO JOURNAL, 1997, 16 (18) :5509-5519

[15] SUMO-1: ubiquitin gains weight [J].

Johnson, PR ;

Hochstrasser, M .

TRENDS IN CELL BIOLOGY, 1997, 7 (10) :408-413

[16] Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein [J].

Kamitani, T ;

Kito, K ;

Nguyen, HP ;

Yeh, ETH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (45) :28557-28562

[17]

KATO S, 1994, GENE, V150, P243

[18] PRODUCTION AND SEPARATION OF PEPTIDES FROM PROTEINS STAINED WITH COOMASSIE BRILLIANT BLUE R-250 AFTER SEPARATION BY SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

KAWASAKI, H ;

EMORI, Y ;

SUZUKI, K .

ANALYTICAL BIOCHEMISTRY, 1990, 191 (02) :332-336

[19] cul-1 is required for cell cycle exit in C-elegans and identifies a novel gene family [J].

Kipreos, ET ;

Lander, LE ;

Wing, JP ;

He, WW ;

Hedgecock, EM .

CELL, 1996, 85 (06) :829-839

[20] CLONING OF A CDNA WHICH ENCODES A NOVEL UBIQUITIN-LIKE PROTEIN [J].

KUMAR, S ;

YOSHIDA, Y ;

NODA, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 195 (01) :393-399

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