A new NEDD8-ligating system for cullin-4A

被引：213

作者：

Osaka, F

Kawasaki, H

Aida, N

Saeki, M

Chiba, T

Kawashima, S

Tanaka, K

Kato, S ^{[1
]}

机构：

[1] Japan Sci & Technol Corp, JST, Kato Cytoprot Network Project, ERATO,Sagami Chem Res Ctr, Kanagawa 2290012, Japan

[2] Japan Sci & Technol Corp, CREST, Bunkyo Ku, Tokyo 1130021, Japan

[3] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1130021, Japan

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 15期

关键词：

ubiquitin; ubiquitin-like protein; NEDD8; SUMO-1; cullin-4A; cdc53;

D O I：

10.1101/gad.12.15.2263

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an El (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino-and carboxy-terminal regions of El and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.

引用

页码：2263 / 2268

页数：6

共 31 条

[11] Cell cycle: Cull and destroy
Jackson, PK
[J]. CURRENT BIOLOGY, 1996, 6 (10) : 1209 - 1212
[12] THE UBIQUITIN-CONJUGATION SYSTEM
JENTSCH, S
[J]. ANNUAL REVIEW OF GENETICS, 1992, 26 : 179 - 207
[13] Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p
Johnson, ES
Blobel, G
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (43) : 26799 - 26802
[14] The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer
Johnson, ES
Schwienhorst, I
Dohmen, RJ
Blobel, G
[J]. EMBO JOURNAL, 1997, 16 (18) : 5509 - 5519
[15] SUMO-1: ubiquitin gains weight
Johnson, PR
Hochstrasser, M
[J]. TRENDS IN CELL BIOLOGY, 1997, 7 (10) : 408 - 413
[16] Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein
Kamitani, T
Kito, K
Nguyen, HP
Yeh, ETH
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (45) : 28557 - 28562
[17] KATO S, 1994, GENE, V150, P243
[18] PRODUCTION AND SEPARATION OF PEPTIDES FROM PROTEINS STAINED WITH COOMASSIE BRILLIANT BLUE R-250 AFTER SEPARATION BY SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS
KAWASAKI, H
EMORI, Y
SUZUKI, K
[J]. ANALYTICAL BIOCHEMISTRY, 1990, 191 (02) : 332 - 336
[19] cul-1 is required for cell cycle exit in C-elegans and identifies a novel gene family
Kipreos, ET
Lander, LE
Wing, JP
He, WW
Hedgecock, EM
[J]. CELL, 1996, 85 (06) : 829 - 839
[20] CLONING OF A CDNA WHICH ENCODES A NOVEL UBIQUITIN-LIKE PROTEIN
KUMAR, S
YOSHIDA, Y
NODA, M
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 195 (01) : 393 - 399

← 1 2 3 4 →