A new NEDD8-ligating system for cullin-4A

被引：214

作者：

Osaka, F

Kawasaki, H

Aida, N

Saeki, M

Chiba, T

Kawashima, S

Tanaka, K

Kato, S ^{[1
]}

机构：

[1] Japan Sci & Technol Corp, JST, Kato Cytoprot Network Project, ERATO,Sagami Chem Res Ctr, Kanagawa 2290012, Japan

[2] Japan Sci & Technol Corp, CREST, Bunkyo Ku, Tokyo 1130021, Japan

[3] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1130021, Japan

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 15期

关键词：

ubiquitin; ubiquitin-like protein; NEDD8; SUMO-1; cullin-4A; cdc53;

D O I：

10.1101/gad.12.15.2263

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an El (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino-and carboxy-terminal regions of El and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.

引用

页码：2263 / 2268

页数：6

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