Development and characterization of 37 SNP markers for the largemouth bass (Micropterus salmoides) by using PCR-RFLP method

被引：1

作者：

Jiang, Zhou ^{[1
]}

Cui, Jiao ^{[1
]}

Shao, Jiaqi ^{[1
,2
]}

Dong, Chuanju ^{[1
]}

Du, Jinxing ^{[2
]}

Shen, Yubang ^{[3
]}

Li, Shengjie ^{[1
,2
]}

Zhang, Meng ^{[1
,3
]}

Li, Xuejun ^{[1
]}

机构：

[1] Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Henan, Peoples R China

[2] Chinese Acad Fisheries Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, China Minist Agr, Guangzhou 510380, Peoples R China

[3] Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr & Rural Affairs, Shanghai 201306, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2021年 / 13卷 / 04期

关键词：

Micropterus salmoides; SNP; PCR-RFLP; Conservation genetics; GENETIC DIVERSITY;

D O I：

10.1007/s12686-021-01229-0

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Largemouth bass (Micropterus salmoides) is an economically important species in China. Contrary to its rapidly increasing yield during the last decades, the domestic genetic diversity of largemouth bass has gradually declined. In this study, 37 single nucleotide polymorphism (SNP) markers were developed based on genotyping-by-sequencing data and characterized by genotyping 32 individuals using the PCR-restriction fragment length polymorphism method. The effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and polymorphic information content (PIC) of these SNPs ranged from 1.168 to 1.998, 0.156 to 0.844, 0.146 to 0.507, and 0.134 to 0.375, respectively. Totally, five loci deviated significantly from Hardy-Weinberg equilibrium (p < 0.05), while there existed no linkage disequilibrium at all loci. These novel polymorphic markers will lay the foundation for future rationally excavation and utilization of germplasm resources in M. salmoides.

引用

页码：429 / 433

页数：5

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