Newly generated interspecific wine yeast hybrids introduce flavour and aroma diversity to wines

被引:73
作者
Bellon, Jennifer R. [1 ]
Eglinton, Jeffery M. [1 ]
Siebert, Tracey E. [1 ]
Pollnitz, Alan P. [2 ]
Rose, Louisa [3 ]
Lopes, Miguel de Barros [4 ]
Chambers, Paul J. [1 ]
机构
[1] Australian Wine Res Inst, Glen Osmond, SA 5064, Australia
[2] Forens Sci S Australia, Adelaide, SA 5000, Australia
[3] Yalumba, Angaston, SA 5353, Australia
[4] Univ S Australia, Sch Pharm & Med Sci, Adelaide, SA 5000, Australia
关键词
Saccharomyces sensu stricto; Interspecific hybrids; Metabolites; Fermentation products; Wine yeast; SACCHAROMYCES-CEREVISIAE; NATURAL HYBRIDS; STRAINS; IDENTIFICATION; GENE; FERMENTATIONS; KUDRIAVZEVII; EVOLUTION;
D O I
10.1007/s00253-011-3294-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Increasingly, winemakers are looking for ways to introduce aroma and flavour diversity to their wines as a means of improving style and increasing product differentiation. While currently available commercial yeast strains produce consistently sound fermentations, there are indications that sensory complexity and improved palate structure are obtained when other species of yeast are active during fermentation. In this study, we explore a strategy to increase the impact of non-Saccharomyces cerevisiae inputs without the risks associated with spontaneous fermentations, through generating interspecific hybrids between a S. cerevisiae wine strain and a second species. For our experiments, we used rare mating to produce hybrids between S. cerevisiae and other closely related yeast of the Saccharomyces sensu stricto complex. These hybrid yeast strains display desirable properties of both parents and produce wines with concentrations of aromatic fermentation products that are different to what is found in wine made using the commercial wine yeast parent. Our results demonstrate, for the first time, that the introduction of genetic material from a non-S. cerevisiae parent into a wine yeast background can impact favourably on the wine flavour and aroma profile of a commercial S. cerevisiae wine yeast.
引用
收藏
页码:603 / 612
页数:10
相关论文
共 40 条
[1]  
[Anonymous], 2012, Molecular Cloning: A Laboratory Manual
[2]  
Ausubel FM., 1994, Curr. Protoc. Mol. Biol
[3]   Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii [J].
Belloch, Carmela ;
Perez-Torrado, Roberto ;
Gonzalez, Sara S. ;
Perez-Ortin, Jose E. ;
Garcia-Martinez, Jose ;
Querol, Amparo ;
Barrio, Eladio .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (08) :2534-2544
[4]   Divergence of transcription factor binding sites across related yeast species [J].
Borneman, Anthony R. ;
Gianoulis, Tara A. ;
Zhang, Zhengdong D. ;
Yu, Haiyuan ;
Rozowsky, Joel ;
Seringhaus, Michael R. ;
Wang, Lu Yong ;
Gerstein, Mark ;
Snyder, Michael .
SCIENCE, 2007, 317 (5839) :815-819
[5]   A homozygous diploid subset of commercial wine yeast strains [J].
Bradbury, John E. ;
Richards, Keith D. ;
Niederer, Heather A. ;
Lee, Soon A. ;
Dunbar, P. Rod ;
Gardner, Richard C. .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2006, 89 (01) :27-37
[6]   Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianus [J].
Dunn, Barbara ;
Sherlock, Gavin .
GENOME RESEARCH, 2008, 18 (10) :1610-1623
[7]   Decreasing acetic acid accumulation by a glycerol overproducing strain of Saccharomyces cerevisiae by deleting the ALD6 aldehyde dehydrogenase gene [J].
Eglinton, JM ;
Heinrich, AJ ;
Pollnitz, AP ;
Langridge, P ;
Henschke, PA ;
Lopes, MD .
YEAST, 2002, 19 (04) :295-301
[8]   Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers [J].
Esteve-Zarzoso, B ;
Belloch, C ;
Uruburu, F ;
Querol, A .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :329-337
[9]  
Fleet G., 1993, WINE MICROBIOLOGY BI, P27
[10]   Natural hybrids from Saccharomyces cerevisiae, Saccharomyces bayanus and Saccharomyces kudriavzevii in wine fermentations [J].
Gonzalez, Sara S. ;
Barrio, Eladio ;
Gafner, Juerg ;
Querol, Amparo .
FEMS YEAST RESEARCH, 2006, 6 (08) :1221-1234