Evaluation of hemocompatibility and in vitro immersion on microwave-assisted hydroxyapatite-alumina nanocomposites

被引：66

作者：

Radha, G. ^{[1
]}

Balakumar, S. ^{[1
]}

Venkatesan, Balaji ^{[2
]}

Vellaichamy, Elangovan ^{[2
]}

机构：

[1] Univ Madras, Natl Ctr Nanosci & Nanotechnol, Madras 600025, Tamil Nadu, India

[2] Univ Madras, Dept Biochem, Madras 600025, Tamil Nadu, India

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2015年 / 50卷

关键词：

Nano-hydroxyapatite; Alumina; Mechanical strength; In vitro bioactivity; Hemocompatibility; Cell proliferation; MECHANICAL-PROPERTIES; TRICALCIUM PHOSPHATE; BONE; COMPOSITE; SCAFFOLDS; RAMAN; BIOCOMPATIBILITY; NANOPARTICLES;

D O I：

10.1016/j.msec.2015.01.054

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

This study reports the microwave-assisted synthesis and characterization of nHAp (nano-hydroxyapatite)alumina composites. The crystalline phase and interaction of alumina with nHAp was analyzed using X-ray diffraction (XRD) and Raman microscopy analysis, respectively. High resolution transmission electron microscopy (HRTEM) micrographs exhibit morphological changes of nHAp composites with increasing alumina concentrations. Microhardness studies reveal the enhanced mechanical strength of nHAp10 and nHAp20 nanocomposites than pure nHAp. In vitro bioactivity of the nanocomposites was studied by immersing samples in simulated body fluid (Hank's solution) for 21 days. The surface of biomineralized samples were analyzed using field emission scanning electron microscopy (FFSEM) and energy dispersive X-ray spectroscopy (EDX). Hemolytic assay revealed acceptable compatibility for varying concentrations of all the samples. Cell proliferation assay was systematically investigated for 1 day and 3 days on Saos-2 osteoblast-like cell lines and it was found that nHAp nanocomposites improved the proliferation. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：143 / 150

页数：8

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