共 39 条
Kinetic analysis of protein aggregation monitored by real-time 2D solid-state NMR spectroscopy
被引:5
作者:

Etzkorn, Manuel
论文数: 0 引用数: 0
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机构:
Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 Utrecht, Netherlands

Boeckmann, Anja
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h-index: 0
机构:
Univ Lyon, CNRS, UMR 5086, Inst Biol & Chim Prot,UCBL, F-69367 Lyon, France Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 Utrecht, Netherlands

Baldus, Marc
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 Utrecht, Netherlands
Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 Utrecht, Netherlands
机构:
[1] Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3584 Utrecht, Netherlands
[2] Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany
[3] Univ Lyon, CNRS, UMR 5086, Inst Biol & Chim Prot,UCBL, F-69367 Lyon, France
关键词:
Aggregation;
Kinetic;
Solid-state NMR;
Real-time spectroscopy;
Crh;
HIGH-RESOLUTION;
MECHANISM;
DYNAMICS;
INTERMEDIATE;
ASSOCIATION;
NUCLEATION;
ACTIN;
C-13;
D O I:
10.1007/s10858-011-9468-6
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
It is shown that real-time 2D solid-state NMR can be used to obtain kinetic and structural information about the process of protein aggregation. In addition to the incorporation of kinetic information involving intermediate states, this approach can offer atom-specific resolution for all detectable species. The analysis was carried out using experimental data obtained during aggregation of the 10.4 kDa Crh protein, which has been shown to involve a partially unfolded intermediate state prior to aggregation. Based on a single real-time 2D C-13-C-13 transition spectrum, kinetic information about the refolding and aggregation step could be extracted. In addition, structural rearrangements associated with refolding are estimated and several different aggregation scenarios were compared to the experimental data.
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收藏
页码:121 / 129
页数:9
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