Anti-inflammatory effect of methyl dehydrojasmonate (J2) is mediated by the NF-κB pathway

被引：34

作者：

Lee, Hye-Ja ^{[1
]}

Maeng, Kyungah ^{[2
,3
]}

Dang, Hung-The ^{[4
]}

Kang, Gyeoung-Jin ^{[1
]}

Ryou, Chongsuk ^{[5
]}

Jung, Jee H. ^{[4
]}

Kang, Hee-Kyoung ^{[1
]}

Prchal, Josef T. ^{[2
,3
]}

Yoo, Eun-Sook ^{[1
]}

Yoon, Donghoon ^{[2
,3
]}

机构：

[1] Cheju Natl Univ, Dept Pharmacol, Coll Med, Cheju 690756, South Korea

[2] VAH, Salt Lake City, UT 84132 USA

[3] Univ Utah, Div Hematol, Salt Lake City, UT 84132 USA

[4] Pusan Natl Univ, Coll Pharm, Pusan 609735, South Korea

[5] Univ Kentucky, Dept Microbiol Immunol & Mol Genet, Lexington, KY 40536 USA

来源：

JOURNAL OF MOLECULAR MEDICINE-JMM | 2011年 / 89卷 / 01期

关键词：

Inflammation; Jasmonate; miRNAs; NF-kappa B pathway; INFLAMMATORY RESPONSE; ANTICANCER AGENTS; CELL-LINE; INDUCTION; CANCER; ACTIVATION; EXPRESSION; PROTEINS; FAMILY; LPS;

D O I：

10.1007/s00109-010-0688-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Inflammation as a major defense mechanism against pathogens is modulated by diverse microbial products. A variety of plant and microbial products interacting with Toll-like receptors initiate a wide spectrum of responses from phagocytosis to cytokine production, which modulates inflammation. Jasmonates are fatty acid-derived cyclopentanones produced by plants and lower eukaryotes that play an important role in the defense against insects. In this study, we are set up to define the molecular targets of J2 action. While the lipopolysaccharide (LPS) stimulation of macrophage cell line RAW264.7 induced TNF-alpha, IL-6, iNOS, and COX-2 that were associated with an increase in miR-155 and miR-146a, the J2 suppressed the induction of these inflammatory cytokines and enzymes as well as miR-155 in a dose-dependent manner. To assess the associations of miR-155 with inflammatory markers, we overexpressed miR-155 and found attenuation of COX-2 suppression with J2 treatment. Furthermore, J2 inhibited NF-kappa B, p65, and I kappa B but had no or only minimal effects on the mitogen-activated protein kinase pathway. In conclusion, the present study demonstrates that J2 suppresses LPS stimulation of RAW264.7 cells by targeting NF-kappa B pathways.

引用

页码：83 / 90

页数：8