Using pot experiment to study the effect of vermicopost on the activities of soil urease, acid invertase, acid phosphatase and catalase of coastal saline soil in water spinach plantation under conditions without chemical fertilizer utilization. Six treatments were designed by mixing vermicompost and saline soil in different volume ratios of 0:20, 2:20, 3:20, 4:20, 5:20 and 6:20. The results showed that adding vermicompost could significantly increase the activities of four kinds of soil enzyme in water spinach plantation. And the activities of urease, acid invertase, acid phosphatase and catalase were increased by 245.9% similar to 378.7%, 30.6% similar to 156.8%, 3.6% similar to 21.7% and 36.3% similar to 147.7%, respectively. The promoting effect of different treatments on soil enzyme activity showed different rules. The activities of urease, acid invertase, acid phosphatase and catalase were characterized by 4:20 > 5:20 > 6:20 > 3:20 > 2:20, 4:20 > 5:20 > 3:20 > 6:20 > 2:20, 5:20 > 4:20 > 3:20 > 2:20 > 6:20 and 6:20 > 5:20 > 4:20 > 3:20 > 2:20, respectively. This indicated that the improving effect of vermicopost on the coastal saline soil was the best in the treatments with volume ratios of 4:20 similar to 5:20.