Blockade of dengue virus transmission from viremic blood to Aedes aegypti mosquitoes using human monoclonal antibodies

被引：2

作者：

Trung Tuan Vu ^{[1
,2
]}

Clapham, Hannah ^{[1
,2
]}

Van Thi Thuy Huynh ^{[1
]}

Long Vo Thi ^{[1
]}

Dui Le Thi ^{[1
]}

Nhu Tuyet Vu ^{[1
]}

Giang Thi Nguyen ^{[1
]}

Trang Thi Xuan Huynh ^{[1
]}

Kien Thi Hue Duong ^{[1
]}

Vi Thuy Tran ^{[1
]}

Huy Le Anh Huynh ^{[1
]}

Duyen Thi Le Huynh ^{[1
]}

Thuy Le Phuong Huynh ^{[1
]}

Thuy Thi Van Nguyen ^{[1
]}

Nguyet Minh Nguyen ^{[1
]}

Tai Thi Hue Luong ^{[3
]}

Nguyen Thanh Phong ^{[3
]}

Chau Van Vinh Nguyen ^{[3
]}

Gough, Gerald ^{[4
]}

Wills, Bridget ^{[1
,2
]}

Carrington, Lauren B. ^{[1
,2
]}

Simmons, Cameron P. ^{[1
,2
,5
]}

机构：

[1] Univ Oxford, Clin Res Unit, Dist 5, Ho Chi Minh City, Vietnam

[2] Univ Oxford, Ctr Trop Med & Global Hlth, Oxford, England

[3] Hosp Trop Dis, Dist 5, Ho Chi Minh City, Vietnam

[4] GlaxoSmithKline, Biopharm Discovery, Stevenage, Herts, England

[5] Monash Univ, Inst Vector Borne Dis, Clayton, Vic, Australia

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2019年 / 13卷 / 11期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

NEUTRALIZING ANTIBODIES; INFECTION; VACCINE;

D O I：

10.1371/journal.pntd.0007142

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background Dengue is the most prevalent arboviral disease of humans. Virus neutralizing antibodies are likely to be critical for clinical immunity after vaccination or natural infection. A number of human monoclonal antibodies (mAbs) have previously been characterized as able to neutralize the infectivity of dengue virus (DENV) for mammalian cells in cell-culture systems. Methodology/Principle findings We tested the capacity of 12 human mAbs, each of which had previously been shown to neutralize DENV in cell-culture systems, to abrogate the infectiousness of dengue patient viremic blood for mosquitoes. Seven of the twelve mAbs (1F4, 14c10, 2D22, 1L12, 5J7, 747(4)B7, 753(3)C10), almost all of which target quaternary epitopes, inhibited DENV infection of Ae. aegypti. The mAbs 14c10, 747(4)B7 and 753(3)C10 could all inhibit transmission of DENV in low microgram per mL concentrations. An Fc-disabled variant of 14c10 was as potent as its parent mAb. Author summary Dengue is the most prevalent arboviral disease affecting humans. There are no therapeutics for the disease. We developed a novel virus neutralization assay, employing Ae. aegypti mosquitoes and viremic blood from dengue patients, to examine the virus-neutralizing potency of 12 human-derived monoclonal antibodies (mAbs) that had previously been shown to neutralize DENV in cell-culture systems. Five of the twelve mAbs failed to block dengue virus infections of mosquitoes using our assay. The remaining seven mAbs neutralized at least one serotype of dengue virus. The results demonstrate that some mAbs were functional and potently neutralized DENV in the complex matrix of viremic human blood. These findings advance the understanding of the types of antibodies that would be desirable to elicit using a dengue vaccine or to apply for acute therapy.

引用

页数：12

共 26 条

[1] The Human Immune Response to Dengue Virus Is Dominated by Highly Cross-Reactive Antibodies Endowed with Neutralizing and Enhancing Activity [J].

Beltramello, Martina ;

Williams, Katherine L. ;

Simmons, Cameron P. ;

Macagno, Annalisa ;

Simonelli, Luca ;

Quyen, Nguyen Than Ha ;

Sukupolvi-Petty, Soila ;

Navarro-Sanchez, Erika ;

Young, Paul R. ;

de Silva, Aravinda M. ;

Rey, Felix A. ;

Varani, Luca ;

Whitehead, Stephen S. ;

Diamond, Michael S. ;

Harris, Eva ;

Lanzavecchia, Antonio ;

Sallusto, Federica .

CELL HOST & MICROBE, 2010, 8 (03) :271-283

[2] The global distribution and burden of dengue [J].

Bhatt, Samir ;

Gething, Peter W. ;

Brady, Oliver J. ;

Messina, Jane P. ;

Farlow, Andrew W. ;

Moyes, Catherine L. ;

Drake, John M. ;

Brownstein, John S. ;

Hoen, Anne G. ;

Sankoh, Osman ;

Myers, Monica F. ;

George, Dylan B. ;

Jaenisch, Thomas ;

Wint, G. R. William ;

Simmons, Cameron P. ;

Scott, Thomas W. ;

Farrar, Jeremy J. ;

Hay, Simon I. .

NATURE, 2013, 496 (7446) :504-507

[3] Dengue Virus Neutralizing Antibody Levels Associated with Protection from Infection in Thai Cluster Studies [J].

Buddhari, Darunee ;

Aldstadt, Jared ;

Endy, Timothy P. ;

Srikiatkhachorn, Anon ;

Thaisomboonsuk, Butsaya ;

Klungthong, Chonticha ;

Nisalak, Ananda ;

Khuntirat, Benjawan ;

Jarman, Richard G. ;

Fernandez, Stefan ;

Thomas, Stephen J. ;

Scott, Thomas W. ;

Rothman, Alan L. ;

Yoon, In-Kyu .

PLOS NEGLECTED TROPICAL DISEASES, 2014, 8 (10)

[4] Field-and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes [J].

Carrington, Lauren B. ;

Bich Chau Nguyen Tran ;

Nhat Thanh Hoang Le ;

Tai Thi Hue Luong ;

Truong Thanh Nguyen ;

Phong Thanh Nguyen ;

Chau Van Vinh Nguyen ;

Huong Thi Cam Nguyen ;

Trung Tuan Vu ;

Long Thi Vo ;

Dui Thi Le ;

Nhu Tuyet Vu ;

Giang Thi Nguyen ;

Hung Quoc Luu ;

Anh Duc Dang ;

Hurst, Timothy P. ;

O'Neill, Scott L. ;

Vi Thuy Tran ;

Duong Thi Hue Kien ;

Nguyet Minh Nguyen ;

Wolbers, Marcel ;

Wills, Bridget ;

Simmons, Cameron P. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (02) :361-366

[5] Identification of human neutralizing antibodies that bind to complex epitopes on dengue virions [J].

de Alwis, Ruklanthi ;

Smith, Scott A. ;

Olivarez, Nicholas P. ;

Messer, William B. ;

Huynh, Jeremy P. ;

Wahala, Wahala M. P. B. ;

White, Laura J. ;

Diamond, Michael S. ;

Baric, Ralph S. ;

Crowe, James E., Jr. ;

de Silva, Aravinda M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (19) :7439-7444

[6] A new class of highly potent, broadly neutralizing antibodies isolated from viremic patients infected with dengue virus [J].

Dejnirattisai, Wanwisa ;

Wongwiwat, Wiyada ;

Supasa, Sunpetchuda ;

Zhang, Xiaokang ;

Dai, Xinghong ;

Rouvinsky, Alexander ;

Jumnainsong, Amonrat ;

Edwards, Carolyn ;

Nguyen Than Ha Quyen ;

Duangchinda, Thaneeya ;

Grimes, Jonathan M. ;

Tsai, Wen-Yang ;

Lai, Chih-Yun ;

Wang, Wei-Kung ;

Malasit, Prida ;

Farrar, Jeremy ;

Simmons, Cameron P. ;

Zhou, Z. Hong ;

Rey, Felix A. ;

Mongkolsapaya, Juthathip ;

Screaton, Gavin R. .

NATURE IMMUNOLOGY, 2015, 16 (02) :170-+

[7] Cross-Reacting Antibodies Enhance Dengue Virus Infection in Humans [J].

Dejnirattisai, Wanwisa ;

Jumnainsong, Amonrat ;

Onsirisakul, Naruthai ;

Fitton, Patricia ;

Vasanawathana, Sirijitt ;

Limpitikul, Wannee ;

Puttikhunt, Chunya ;

Edwards, Carolyn ;

Duangchinda, Thaneeya ;

Supasa, Sunpetchuda ;

Chawansuntati, Kriangkrai ;

Malasit, Prida ;

Mongkolsapaya, Juthathip ;

Screaton, Gavin .

SCIENCE, 2010, 328 (5979) :745-748

[8] Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers [J].

Fibriansah, Guntur ;

Ibarra, Kristie D. ;

Thiam-Seng Ng ;

Smith, Scott A. ;

Tan, Joanne L. ;

Lim, Xin-Ni ;

Ooi, Justin S. G. ;

Kostyuchenko, Victor A. ;

Wang, Jiaqi ;

de Silva, Aravinda M. ;

Harris, Eva ;

Crowe, James E., Jr. ;

Lok, Shee-Mei .

SCIENCE, 2015, 349 (6243) :88-91

[9] A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins [J].

Fibriansah, Guntur ;

Tan, Joanne L. ;

Smith, Scott A. ;

de Alwis, Ruklanthi ;

Ng, Thiam-Seng ;

Kostyuchenko, Victor A. ;

Jadi, Ramesh S. ;

Kukkaro, Petra ;

de Silva, Aravinda M. ;

Crowe, James E. ;

Lok, Shee-Mei .

NATURE COMMUNICATIONS, 2015, 6

[10] A potent anti-dengue human antibody preferentially recognizes the conformation of E protein monomers assembled on the virus surface [J].

Fibriansah, Guntur ;

Tan, Joanne L. ;

Smith, Scott A. ;

de Alwis, Adamberage R. ;

Thiam-Seng Ng ;

Kostyuchenko, Victor A. ;

Ibarra, Kristie D. ;

Wang, Jiaqi ;

Harris, Eva ;

de Silva, Aravinda ;

Crowe, James E., Jr. ;

Lok, Shee-Mei .

EMBO MOLECULAR MEDICINE, 2014, 6 (03) :358-371

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