Fluorescence lifetime tomography of live cells expressing enhanced green fluorescent protein embedded in a scattering medium exhibiting background autofluorescence

被引：23
作者：
Soloviev, Vadim Y.
McGinty, James
Tahir, Khadija B.
Neil, Mark A. A.
Sardini, Alessandro
Hajnal, Joseph V.
Arridge, Simon R.
French, P. M. W.
机构：
[1] UCL, Dept Comp Sci, London WC1E 6BT, England
[2] Imperial Coll Photon, Blackett Lab, London SW7 2BW, England
[3] Imperial Coll Sch Med, Fac Med, MRC Clin Sci Ctr, London W12 0NN, England
来源：
OPTICS LETTERS | 2007年 / 32卷 / 14期
关键词：
D O I：
10.1364/OL.32.002034
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We present a novel fluorescence lifetime tomography system applied to a highly scattering autofluorescent phantom containing live cells expressing the fluorophore enhanced green fluorescent protein (EGFP). The fluorescence signal was excited using a fiber-laser-pumped supercontinuum source and detected using wide-field time gating imaging. To facilitate rapid 3D reconstruction of the fluorescence lifetime distribution, the time-resolved data were Fourier-transformed in time to give complex functions that formed a data set for the Fourier domain reconstruction. Initially the presence of an unspecified background autofluorescence signal impeded reconstruction of the lifetime distribution, but we show that this problem can be addressed using a simple iterative technique. (C) 2007 Optical Society of America.
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收藏
页码：2034 / 2036
页数：3
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