Fast Screening and Secure Confirmation of Milk Powder Adulteration with Maltodextrin via Electrospray Ionization-Mass Spectrometry [ESI(+)-MS] and Selective Enzymatic Hydrolysis

被引:16
作者
Sanvido, Gustavo B. [1 ]
Garcia, Jerusa S. [1 ]
Corilo, Yuri E. [1 ]
Vaz, Boniek G. [1 ]
Zacca, Jorge J. [2 ]
Cosso, Ricardo G.
Eberlin, Marcos N. [1 ]
Peter, Martin G. [1 ]
机构
[1] Univ Campinas UNICAMP, Inst Chem, Thomson Mass Spectrometry Lab, BR-13083970 Campinas, SP, Brazil
[2] Natl Inst Criminalist, Minist Justice, Brazilian Fed Police Dept, BR-70610200 Distrito Fed, Brazil
基金
巴西圣保罗研究基金会;
关键词
ESI-MS; fraud; glycosidases; lactose; maltodextrin; mass spectrometry; milk powder; BUFFALO MOZZARELLA; EWE CHEESE; COW MILK; CHROMATOGRAPHY; AUTHENTICITY; QUALITY; RATIO; TOOL;
D O I
10.1021/jf1014445
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Direct-infusion electrospray ionization-mass spectrometry [ESI(+)-MS] of several milk powder samples, confiscated by the Brazilian Federal Police, showed ions accounting for sodiated and potassiated molecules of disaccharides (m/z 365 and 381) as well as trisaccharides (m/z 527 and 543), whereas monosaccharide ions were not detected. The trisaccharide ions were not detected in samples of genuine milk powder, raising the suspicion that their presence indicates adulteration by the addition of maltodextrin. In control samples, maltose and maltotriose were hydrolyzed by a-glucosidase and not beta-galactosidase, whereas lactose was resistant to alpha-glucosidase but was hydrolyzed with beta-galactosidase. Samples suspected of being adulterated behaved in the same fashion, confirming the presence of maltose and maltotriose or maltodextrin. Direct-infusion ESI MS is shown therefore to provide rapid screening of milk powder for adulteration with maltodextrin, whereas its combination with selective enzymatic hydrolysis provides highly reliable confirmation for unambiguous results.
引用
收藏
页码:9407 / 9412
页数:6
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