Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma

被引：71
作者：
Dyrskjot, L. [1 ]
Zieger, K. [2 ]
Lildal, T. Kissow [2 ]
Reinert, T. [1 ]
Gruselle, O. [3 ]
Coche, T. [3 ]
Borre, M. [2 ]
Orntoft, T. F. [1 ]
机构：
[1] Aarhus Univ Hosp, Dept Mol Med, DK-8200 Aarhus N, Denmark
[2] Aarhus Univ Hosp, Dept Urol, DK-8200 Aarhus N, Denmark
[3] GlaxoSmithKline Biol, B-1330 Rixensart, Belgium
来源：
BRITISH JOURNAL OF CANCER | 2012年 / 107卷 / 01期
关键词：
bladder cancer; CT genes; immunotherapy; PCR; prognosis; CANCER-TESTIS ANTIGENS; BLADDER-CANCER; CANCER/TESTIS ANTIGENS; MELANOMA; GENES; IMMUNOTHERAPY; SURVIVAL; PEPTIDE;
D O I：
10.1038/bjc.2012.215
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
BACKGROUND: The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. METHODS: We used specific q-RT-PCR assays to analyse the expression of the CT genes MAGE-A3, NY-ESO-1 (CTAG1B), LAGE-1 (CTAG2) and PRAME in a panel of bladder tumours from 350 patients with long-term follow-up and detailed treatment information. RESULTS: Overall, 43% of the tumours expressed MAGE-A3, 35% expressed NY-ESO-1, 27% expressed LAGE-1 and 20% expressed PRAME. In all, 56% of the tumours expressed at least one of the CT genes analysed. Univariate Cox regression analysis of CT gene expression in non-muscle-invasive tumours showed that expression of MAGE-A3 (P = 0.026), LAGE-1 (P = 0.001) and NY-ESO-1 (P = 0.040) was significantly associated with a shorter progression-free survival. In addition, we found that patients with tumours expressing PRAME responded poorly to chemotherapy (P = 0.02, chi(2)-test). CONCLUSION: Cancer-testis genes are frequently expressed in bladder cancer and especially in tumours of high stage and grade. In addition, the CT gene expression may have both prognostic and predictive value. Development of specific immunotherapy against the CT antigens in bladder cancer may ultimately increase patient survival. British Journal of Cancer (2012) 107, 116-122. doi: 10.1038/bjc.2012.215 www.bjcancer.com Published online 17 May 2012 (C) 2012 Cancer Research UK
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页码：116 / 122
页数：7
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