Formulation and Characterization of Doxycycline-Loaded Polymeric Nanoparticles for Testing Antitumor/Antiangiogenic Action in Experimental Colon Cancer in Mice

被引:16
作者
Alshaman, Reem [1 ]
Alattar, Abdullah [1 ]
El-Sayed, Rehab M. [2 ]
Gardouh, Ahmed R. [3 ,4 ]
Elshaer, Rabie E. [5 ]
Elkazaz, Amany Y. [6 ,7 ]
Eladl, Mohamed Ahmed [8 ]
El-Sherbiny, Mohamed [9 ]
Farag, Noha E. [10 ,11 ]
Hamdan, Ahmed Mohsen [12 ]
Zaitone, Sawsan A. [13 ]
机构
[1] Univ Tabuk, Fac Pharm, Dept Pharmacol & Toxicol, Tabuk 71491, Saudi Arabia
[2] Sinai Univ, Fac Pharm, Dept Pharmacol & Toxicol, Al Arish 45511, Egypt
[3] Suez Canal Univ, Fac Pharm, Dept Pharmaceut & Ind Pharm, Ismailia 41522, Egypt
[4] Jadara Univ, Fac Pharm, Dept Pharm, Irbid 21110, Jordan
[5] Al Azhar Univ, Fac Med, Dept Pathol, Cairo 11651, Egypt
[6] Suez Canal Univ, Fac Med, Biochem & Mol Biol Dept, Ismailia 41522, Egypt
[7] Port Said Univ, Fac Med, Biochem & Mol Biol Dept, Port Said 42526, Egypt
[8] Univ Sharjah, Basic Med Sci Dept, Sharjah 27272, U Arab Emirates
[9] Al Maarefa Univ, Coll Med, Dept Basic Med Sci, Riyadh 71666, Saudi Arabia
[10] Suez Canal Univ, Fac Med, Dept Physiol, Ismailia 41522, Egypt
[11] Taif Univ, Coll Med, Dept Physiol, At Taif 21974, Saudi Arabia
[12] Univ Tabuk, Fac Pharm, Dept Pharm Practice, Tabuk 71491, Saudi Arabia
[13] Suez Canal Univ, Fac Pharm, Dept Pharmacol & Toxicol, Ismailia 41522, Egypt
关键词
angiogenesis; mouse colon cancer; eudragit S100; hydroxypropyl methylcellulose phthalate; doxycycline polymeric nanoparticles; nanoprecipitation method; ENDOTHELIAL GROWTH-FACTOR; DRUG-DELIVERY; IN-VITRO; ANTIBACTERIAL ACTIVITY; STEM-CELLS; TUMOR; VEGF; ANGIOGENESIS; COMBINATION; EXPRESSION;
D O I
10.3390/nano12050857
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Nanotherapeutics can enhance the characteristics of drugs, such as rapid systemic clearance and systemic toxicities. Polymeric nanoparticles (PRNPs) depend on dispersion of a drug in an amorphous state in a polymer matrix. PRNPs are capable of delivering drugs and improving their safety. The primary goal of this study is to formulate doxycycline-loaded PRNPs by applying the nanoprecipitation method. Eudragit S100 (ES100) (for DOX-PRNP1) and hydroxypropyl methyl cellulose phthalate HP55 (for DOX-PRNP2) were tested as the drug carrying polymers and the DOX-PRNP2 showed better characteristics and drug release % and was hence selected to be tested in the biological study. Six different experimental groups were formed from sixty male albino mice. 1,2,-Dimethylhydrazine was used for 16 weeks to induce experimental colon cancer. We compared the oral administration of DOX-PRNP2 in doses of 5 and 10 mg/kg with the free drug. Results indicated that DOX-PRNP2 had greater antitumor activity, as evidenced by an improved histopathological picture for colon specimens as well as a decrease in the tumor scores. In addition, when compared to free DOX, the DOX-PRNP2 reduced the angiogenic indicators VEGD and CD31 to a greater extent. Collectively, the findings demonstrated that formulating DOX in PRNPs was useful in enhancing antitumor activity and can be used in other models of cancers to verify their efficacy and compatibility with our study.
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页数:20
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