Serotonin Signaling Is Required for Wnt-Dependent GRP Specification and Leftward Flow in Xenopus

被引:49
作者
Beyer, Tina [1 ]
Danilchik, Michael [2 ]
Thumberger, Thomas [1 ]
Vick, Philipp [1 ]
Tisler, Matthias [1 ]
Schneider, Isabelle [1 ]
Bogusch, Susanne [1 ]
Andre, Philipp [1 ]
Ulmer, Baerbel [1 ]
Walentek, Peter [1 ]
Niesler, Beate [3 ]
Blum, Martin [1 ]
Schweickert, Axel [1 ]
机构
[1] Univ Hohenheim, Inst Zool, D-70593 Stuttgart, Germany
[2] Oregon Hlth & Sci Univ, Dept Integrat Biosci, Portland, OR 97239 USA
[3] Univ Heidelberg Hosp, Dept Human Mol Genet, D-69120 Heidelberg, Germany
基金
美国国家科学基金会;
关键词
LEFT-RIGHT AXIS; LEFT-RIGHT ASYMMETRY; BETA-CATENIN; NODAL CILIA; EARLY STEP; EXPRESSION; ESTABLISHMENT; ATPASE; MORPHOGENESIS; SYMMETRY;
D O I
10.1016/j.cub.2011.11.027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In vertebrates, most inner organs are asymmetrically arranged with respect to the main body axis [1]. Symmetry breakage in fish, amphibian, and mammalian embryos depends on cilia-driven leftward flow of extracellular fluid during neurulation [2-5]. Flow induces the asymmetric nodal cascade that governs asymmetric organ morphogenesis and placement [1, 6, 7]. In the frog Xenopus, an alternative laterality-generating mechanism involving asymmetric localization of serotonin at the 32-cell stage has been proposed [8]. However, no functional linkage between this early localization and flow at neurula stage has emerged. Here, we report that serotonin signaling is required for specification of the superficial mesoderm (SM), which gives rise to the ciliated gastrocoel roof plate (GRP) where flow occurs [5, 9]. Flow and asymmetry were lost in embryos in which serotonin signaling was downregulated. Serotonin, which we found uniformly distributed along the main body axes in the early embryo, was required for Wnt signaling, which provides the instructive signal to specify the GRP. Importantly, serotonin was required for Wnt-induced double-axis formation as well. Our data confirm flow as primary mechanism of symmetry breakage and suggest a general role of serotonin as competence factor for Wnt signaling during axis formation in Xenopus.
引用
收藏
页码:33 / 39
页数:7
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