Endothelial-dependent vasodilation is associated with increases in the phosphorylation of a small heat shock protein (HSP20)

被引:28
|
作者
Jerius, H
Karolyi, DR
Mondi, JS
Beall, A
Wootton, D
Ku, D
Cable, S
Brophy, CM
机构
[1] Med Coll Georgia, Dept Surg, Augusta, GA 30912 USA
[2] Med Coll Georgia, Dept Med, Inst Mol Med & Genet, Augusta, GA 30912 USA
[3] Med Coll Georgia, Dept Cell Biol & Anat, Augusta, GA 30912 USA
[4] Augusta Vet Adm Med Ctr, Augusta, GA USA
[5] Georgia Inst Technol, Vasc & Biofluids Res Grp, Augusta, GA USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/S0741-5214(99)70314-9
中图分类号
R61 [外科手术学];
学科分类号
摘要
Purpose: Increases in the phosphorylation of a small heat shock protein (HSP20) are associated with cyclic nucleotide-dependent vasorelaxation. The effect-of pressure and now on vessel diameter was studied. We hypothesized that physiologic conditions that induce vasorelaxation would lead to increases in HSP20 phosphorylation. Methods: Flow-dependent changes in vessel diameter, at different intraluminal pressures, were measured with a laser optical micrometer in intact bovine carotid arteries. Experiments were performed in the presence and absence of norepinephrine (10(-5) moI/L). Increases in the phosphorylation of HSP20 were determined with isoelectric focusing immunoblots. Results: The increase in vessel diameter was most significant at low intraluminal pressures (20 mm Hg), high flow rates (200 mL/min), and in the presence of the vasoconstrictor norepinephrine (10(-5) mol/L). The addition of methylene blue (a guanylate cyclase inhibitor) completely inhibited now-induced vasodilation. Under conditions in which maximal flow induced vasodilation occurred, there were significant-increases in the phosphorylation of HSP20. Conclusion: Flow-dependent vasodilation in isolated perfused segments of bovine carotid arteries was maximal when the intraluminal pressures were low and when the vessels were precontracted with norepinephrine. Flow-dependent vasodilation was inhibited by methylene blue and was associated with increases in the phosphorylation of HSP20, suggesting, that the vasodilation was mediated by endothelial production of nitric oxide.
引用
收藏
页码:678 / 684
页数:7
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