Microfluidics for Applications in Cell Mechanics and Mechanobiology

被引:29
|
作者
Hou, Han Wei [1 ,2 ]
Lee, Wong Cheng [2 ,3 ]
Leong, Man Chun [3 ,4 ]
Sonam, Surabhi [4 ]
Vedula, Sri Ram Krishna [4 ]
Lim, Chwee Teck [1 ,3 ,4 ,5 ]
机构
[1] Natl Univ Singapore, Dept Bioengn, Singapore 117576, Singapore
[2] Singapore Alliance Res & Technol SMART Ctr, BioSyst & Micromech BioSyM IRG, Singapore, Singapore
[3] Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore 117576, Singapore
[4] Natl Univ Singapore, Mechanobiol Inst, Singapore 117576, Singapore
[5] Natl Univ Singapore, Dept Mech Engn, Singapore 117576, Singapore
关键词
Cell and molecular mechanics; Mechanobiology; Microfluidics; Microdevices; Human; Diseases; Mechanotransduction; BREAST-CANCER CELLS; FLUID SHEAR-STRESS; PLASMODIUM-FALCIPARUM; SINGLE-CELL; STEM-CELLS; GENE-EXPRESSION; FLOW; BLOOD; SEPARATION; DEFORMABILITY;
D O I
10.1007/s12195-011-0209-4
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Cell mechanics is a highly interdisciplinary research area which has made significant progress over the last decade, particularly in the study of human diseases. In diseases such as malaria and cancer, diseased cells undergo changes in both composition and organization of its cellular structures, which may eventually manifest as changes in the cell mechanical properties such as size and shape, deformability and cell adhesion. Despite the development of state-of-the-art experimental tools to manipulate and probe the cellular mechanical properties, microfluidics has emerged as an enabling technology for study of cell and molecular mechanics due to its numerous inherent advantages including small length scale, reduced sample and reagent volumes and low device cost. This paper presents an overview of the recent efforts in the study of cellular biomechanics using microfluidic approaches. Different areas of studies such as probing of cellular mechanical properties, cell separation using physical biomarkers (size, deformability, density) and effect of shear stress on cellular behavior and responses will be highlighted. Finally, we will discuss on the limitations and challenges associated with current microfluidics-based technologies and offer perspectives for future studies relating to cell biomechanics.
引用
收藏
页码:591 / 602
页数:12
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