Distinct localisation of lipids in the ovarian follicular environment

被引:23
作者
Pantasri, Tawiwan [1 ]
Wu, Linda L. [1 ]
Hull, M. Louise [1 ,2 ]
Sullivan, Thomas R. [3 ]
Barry, Michael [2 ]
Norman, Robert J. [1 ,2 ]
Robker, Rebecca L. [1 ]
机构
[1] Univ Adelaide, Robinson Res Inst, Sch Paediat & Reprod Hlth, Adelaide, SA 5005, Australia
[2] St Andrews Hosp, Fertil SA, Adelaide, SA 5000, Australia
[3] Univ Adelaide, Sch Populat Hlth, Adelaide, SA 5005, Australia
关键词
fatty acid; glucose; insulin; oocyte; IN-VITRO FERTILIZATION; BODY-MASS INDEX; ASSISTED REPRODUCTIVE TREATMENT; FATTY-ACID-COMPOSITION; STIMULATING-HORMONE; INSULIN-RESISTANCE; OBESE WOMEN; OOCYTE; FLUID; OVERWEIGHT;
D O I
10.1071/RD14321
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Obesity is associated with decreased pregnancy rates due, in part, to compromised oocyte quality. The aim of the present cross-sectional study of 84 women undergoing oocyte aspiration was to: (1) compare insulin, lipids and glucose in follicular fluid with serum; (2) determine whether increased body mass index (BMI) and waist circumference, hyperinsulinaemia, dyslipidaemia or metabolic syndrome altered follicular fluid metabolites; and (3) determine relative lipid content in oocytes to reveal any influence of these parameters on oocyte quality and IVF outcomes. Insulin, glucose, triglyceride and free fatty acids were lower in follicular fluid than blood and not strictly correlated between compartments. Insulin, glucose and triglyceride positively correlated with increasing BMI and waist circumference in blood and follicular fluid. Insulin increased in follicular fluid in association with metabolic syndrome. Free fatty acid composition analysis showed saturated fatty acids, particularly palmitic and stearic acid, to be more prevalent in follicular fluid than blood. There were no associations between follicular fluid metabolites or oocyte lipid content and clinical outcomes; however, oocyte immaturity correlated with follicular fluid glucose and fatty acid levels, as well as metabolic syndrome. The present study confirms that the human ovarian follicular environment surrounding the oocyte exhibits a unique metabolite profile compared with blood, with distinct localisation of lipids within follicular fluid and oocytes.
引用
收藏
页码:593 / 601
页数:9
相关论文
共 50 条
  • [21] Female obesity adversely affects assisted reproductive technology (ART) pregnancy and live birth rates
    Luke, Barbara
    Brown, Morton B.
    Stern, Judy E.
    Missmer, Stacey A.
    Fujimoto, Victor Y.
    Leach, Richard
    [J]. HUMAN REPRODUCTION, 2011, 26 (01) : 245 - 252
  • [22] High Fat Diet Induced Developmental Defects in the Mouse: Oocyte Meiotic Aneuploidy and Fetal Growth Retardation/Brain Defects
    Luzzo, Kerri M.
    Wang, Qiang
    Purcell, Scott H.
    Chi, Maggie
    Jimenez, Patricia T.
    Grindler, Natalia
    Schedl, Tim
    Moley, Kelle H.
    [J]. PLOS ONE, 2012, 7 (11):
  • [23] The association between severe obesity and characteristics of failed fertilized oocytes
    Machtinger, Ronit
    Combelles, Catherine M. H.
    Missmer, Stacey A.
    Correia, Katharine F.
    Fox, Janis H.
    Racowsky, Catherine
    [J]. HUMAN REPRODUCTION, 2012, 27 (11) : 3198 - 3207
  • [24] Fatty acid composition of fertilization-failed human oocytes
    Matorras, R
    Ruiz, JI
    Mendoza, R
    Ruiz, N
    Sanjurjo, P
    Rodriguez-Escudero, FJ
    [J]. HUMAN REPRODUCTION, 1998, 13 (08) : 2227 - 2230
  • [25] Effects of polycystic ovarian syndrome on in vitro fertilization-embryo transfer outcomes are influenced by body mass index
    McCormick, Betsy
    Thomas, Michael
    Maxwell, Rose
    Williams, Daniel
    Aubuchon, Mira
    [J]. FERTILITY AND STERILITY, 2008, 90 (06) : 2304 - 2309
  • [26] The role of fatty acids in oocyte and early embryo development
    McKeegan, Paul J.
    Sturmey, Roger G.
    [J]. REPRODUCTION FERTILITY AND DEVELOPMENT, 2012, 24 (01) : 59 - 67
  • [27] Associations Between Insulin Resistance, Free Fatty Acids, and Oocyte Quality in Polycystic Ovary Syndrome During In Vitro Fertilization
    Niu, Zhihong
    Lin, Nan
    Gu, Ruihuan
    Sun, Yijuan
    Feng, Yun
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2014, 99 (11) : E2269 - E2276
  • [28] Metabolic profiling of human follicular fluid identifies potential biomarkers of oocyte developmental competence
    O'Gorman, A.
    Wallace, M.
    Cottell, E.
    Gibney, M. J.
    McAuliffe, F. M.
    Wingfield, M.
    Brennan, L.
    [J]. REPRODUCTION, 2013, 146 (04) : 389 - 395
  • [29] NMR metabolic profile of human follicular fluid
    Pinero-Sagredo, Eva
    Nunes, Sofia
    Jose de los Santos, Ma
    Celda, Bernardo
    Esteve, Vicent
    [J]. NMR IN BIOMEDICINE, 2010, 23 (05) : 485 - 495
  • [30] A Role of Lipid Metabolism during Cumulus-Oocyte Complex Maturation: Impact of Lipid Modulators to Improve Embryo Production
    Prates, E. G.
    Nunes, J. T.
    Pereira, R. M.
    [J]. MEDIATORS OF INFLAMMATION, 2014, 2014