Thiol-functionalized inactivated yeast embedded in agar aerogel for highly efficient adsorption of patulin in apple juice

The issue of patulin (PAT) contamination in apple juice has attracted widespread concern. Recently, inactivated yeast based biosorbents have shown great advantages in the removal of toxic contaminants. However, the traditional yeast adsorbents have disadvantages of a limited adsorption capacity in juice and separation difficulty. In the present work, five chemical thiol-functionalization methods were used to increase the PAT adsorption efficiency of yeast cells in apple juice. Thereinto, glutaraldehyde cross-linking increased the thiol (-SH) content of yeast cells to 1.26 mmol g-1 and improved the PAT adsorption capacity of inactivated yeast in apple juice by 150 times. The covalent bonding of -SH and PAT played an important role in the improvement of adsorption capacity. The as-prepared thiol-modification yeast (Y-SH(Gl)) was then embedded in the agar aerogel to obtain Y-SH(Gl)@Agar free of separation. PAT adsorption of Y-SH(Gl)@Agar was consistent with the Freundlich model and the pseudo-second-order kinetic model. Moreover, Y-SH(Gl)@Agar was competent for PAT removal in apple juice and manifested negligible effects on juice quality. Cytotoxicity investigation indicated its good biocompatibility and ignorable food safety risk, thereby demonstrating that Y-SH(Gl)@Agar may be a promising adsorbent material for the control of PAT contaminant in juice.