A Dinuclear Osmium(II) Complex Near-Infrared Nanoscopy Probe for Nuclear DNA

被引：25

作者：

Droge, Fabian ^{[1
,2
]}

Noakes, Felicity F. ^{[3
,4
]}

Archer, Stuart A. ^{[3
,5
]}

Sreedharan, Sreejesh ^{[3
,6
]}

Raza, Ahtasham ^{[7
,8
]}

Robertson, Craig C. ^{[3
]}

MacNeil, Sheila ^{[7
]}

Haycock, John W. ^{[7
]}

Carson, Heather ^{[3
]}

Meijer, Anthony J. H. M. ^{[3
]}

Smythe, Carl G. W. ^{[4
]}

de la Serna, Jorge Bernardino ^{[9
,10
]}

Dietzek-Ivansic, Benjamin ^{[1
,2
]}

Thomas, Jim A. ^{[3
]}

机构：

[1] Inst Phys Chem, Helmholtzweg 4, D-07743 Jena, Germany

[2] Inst Photon Technol Jena eV, D-07749 Jena, Germany

[3] Univ Sheffield, Dept Chem, Sheffield S10 2TN, S Yorkshire, England

[4] Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England

[5] Loughborough Univ, Dept Chem, Loughborough LE11 3TU, Leics, England

[6] Univ Derby, Sch Human Sci, Kedleston Rd, Derby DE22 1GB, England

[7] Univ Sheffield, Mat Sci & Engn, Sheffield S10 2TN, S Yorkshire, England

[8] XCe11R8 Tech Space, 1 Keckwick Lane, Warrington WA4 4AB, Cheshire, England

[9] Rutherford Appleton Lab, Sci & Technol, Cent Laser Facil, Res Complex & Harwell, Didcot OX11 0QX, Oxon, England

[10] Imperial Coll London, Fac Med, Natl Heart & Lung Inst, London SW7 2AZ, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2021年 / 143卷 / 48期

基金：

英国工程与自然科学研究理事会;

关键词：

RUTHENIUM(II) POLYPYRIDYL COMPLEX; INTERLIGAND ELECTRON-TRANSFER; LIVE-CELL; FLUORESCENT-PROBES; SUPERRESOLUTION MICROSCOPY; ANTICANCER DRUG; CHEMICAL SPACE; GUANINE BASES; FAR-RED; RU;

D O I：

10.1021/jacs.1c10325

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

With the aim of developing photostable near-infrared cell imaging probes, a convenient route to the synthesis of heteroleptic Os-II complexes containing the Os(TAP)(2) fragment is reported. This method was used to synthesize the dinuclear OsII complex, [{Os(TAP)(2)}(2)tpphz](4+) (where tpphz = tetrapyrido[3,2-a:2',3'-c:3",2''-h:2"',3'''-j]phenazine and TAP = 1,4,5,8- tetraaza-phenanthrene). Using a combination of resonance Raman and time-resolved absorption spectroscopy, as well as computational studies, the excited state dynamics of the new complex were dissected. These studies revealed that, although the complex has several close lying excited states, its near-infrared, NIR, emission (lambda(max) = 780 nm) is due to a low-lying Os -> TAP based (MCLT)-M-3 state. Cell-based studies revealed that unlike its Ru-II analogue, the new complex is neither cytotoxic nor photocytotoxic. However, as it is highly photostable as well as live-cell permeant and displays NIR luminescence within the biological optical window, its properties make it an ideal probe for optical microscopy, demonstrated by its use as a super-resolution NIR STED probe for nuclear DNA.

引用

页码：20442 / 20453

页数：12

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