Prediction model for aneuploidy in early human embryo development revealed by single-cell analysis

被引:114
作者
Vera-Rodriguez, Maria [1 ,2 ,3 ]
Chavez, Shawn L. [1 ,2 ]
Rubio, Carmen [3 ,4 ]
Pera, Renee A. Reijo [1 ,2 ]
Simon, Carlos [2 ,3 ,4 ]
机构
[1] Ctr Reprod & Stem Cell Biol, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA
[2] Ctr Reprod & Stem Cell Biol, Inst Stem Cell Biol & Regenerat Med, Dept Obstet & Gynecol, Stanford, CA 94305 USA
[3] Parc Cient Univ Valencia, IGenomix, Valencia 46980, Spain
[4] Univ Valencia, Fdn Inst Valenciano Infertilidad, INCLIVA Hlth Res Inst, Dept Obstet & Gynecol, Valencia 46980, Spain
关键词
HUMAN PREIMPLANTATION EMBRYOS; APOPTOSIS-RELATED GENES; HUMAN OOCYTES; DNA-REPAIR; IMPLANTATION RATES; GENOME ACTIVATION; MESSENGER-RNA; MOUSE OOCYTES; EXPRESSION; BLASTOCYST;
D O I
10.1038/ncomms8601
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Aneuploidies are prevalent in the human embryo and impair proper development, leading to cell cycle arrest. Recent advances in imaging and molecular and genetic analyses are postulated as promising strategies to unveil the mechanisms involved in aneuploidy generation. Here we combine time-lapse, complete chromosomal assessment and single-cell RT-qPCR to simultaneously obtain information from all cells that compose a human embryo until the approximately eight-cell stage (n = 85). Our data indicate that the chromosomal status of aneuploid embryos (n = 26), including those that are mosaic (n = 3), correlates with significant differences in the duration of the first mitotic phase when compared with euploid embryos (n = 28). Moreover, gene expression profiling suggests that a subset of genes is differentially expressed in aneuploid embryos during the first 30 h of development. Thus, we propose that the chromosomal fate of an embryo is likely determined as early as the pronuclear stage and may be predicted by a 12-gene transcriptomic signature.
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页数:14
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