Cloning and characterization of a novel human SPRYD4 gene encoding a putative SPRY domain-containing protein

被引：3

作者：

Zhong, Zhaomin ^{[1
]}

Zhang, Haoxing ^{[1
]}

Bai, Meirong ^{[1
]}

Ni, Jun ^{[1
]}

Wan, Bo ^{[1
]}

Chen, Xinya ^{[1
]}

Yu, Long ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

来源：

DNA SEQUENCE | 2008年 / 19卷 / 01期

基金：

中国国家自然科学基金;

关键词：

B30.2; domain; SPRY domain; SPRYD4; subcellular localization; 12q13.2;

D O I：

10.1080/10425170701433101

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report here the cloning and characterization of a novel human SPRYD4 gene which encodes a SPRY domain containing protein. The SPRYD4 gene is isolated from the human brain cDNA library, and mapped to 12q 13.2 by searching the UCSC genomic database. The SPRYD4 cDNA is 1201 base pairs in length and contains an open reading frame encoding 207 amino acids. The SPRYD4 gene consists of two exons and encodes a putative protein with a SPRY domain ranging from 86 to 203 amino acids. The RT-PCR analysis reveals that SPRYD4 is ubiquitously expressed in 18 human tissues. However, it is strongly expressed in kidney, bladder, brain, thymus and stomach, while weakly expressed liver, testis, uterus, spleen and lung. Subcellular localization demonstrates that SPRYD4 protein is localized in the nuclear when overexpressed in COS-7 cell.

引用

页码：68 / 72

页数：5

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