Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia

被引：57

作者：

Duong, Veasna ^{[1
]}

Mey, Channa ^{[1
]}

Eloit, Marc ^{[2
]}

Zhu, Huachen ^{[3
]}

Danet, Lucie ^{[1
]}

Huang, Zhong ^{[4
]}

Zou, Gang ^{[4
]}

Tarantola, Arnaud ^{[1
]}

Cheval, Justine ^{[5
]}

Perot, Philippe ^{[2
]}

Laurent, Denis ^{[6
]}

Richner, Beat ^{[6
]}

Ky, Santy ^{[6
]}

Heng, Sothy ^{[6
]}

Touch, Sok ^{[7
]}

Sovann, Ly ^{[7
]}

van Doorn, Rogier ^{[8
]}

Thanh Tan Tran ^{[8
]}

Farrar, Jeremy J. ^{[8
]}

Wentworth, David E. ^{[9
]}

Das, Suman R. ^{[9
]}

Stockwell, Timothy B. ^{[9
]}

Manuguerra, Jean-Claude ^{[2
]}

Delpeyroux, Francis ^{[2
,10
]}

Guan, Yi ^{[3
]}

Altmeyer, Ralf ^{[4
]}

Buchy, Philippe ^{[1
,11
]}

机构：

[1] Pasteur Inst Cambodia, Phnom Penh 12000, Cambodia

[2] Pasteur Inst, F-75724 Paris, France

[3] Univ Hong Kong, Sch Publ Hlth, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China

[4] Inst Pasteur Shanghai, Shanghai 200025, Peoples R China

[5] PathoQuest, F-75015 Paris, France

[6] Kantha Bopha Hosp, Phnom Penh 12000, Cambodia

[7] Minist Hlth, Phnom Penh 12000, Cambodia

[8] Univ Oxford, Clin Res Unit, Hosp Trop Dis, Ho Chi Minh P1Q5, Vietnam

[9] J Craig Venter Inst, Rockville, MD 92037 USA

[10] Natl Inst Hlth & Med Res, INSERM, U994, F-75000 Paris, France

[11] GlaxoSmithKline Vaccines R&D, Singapore 189720, Singapore

来源：

EMERGING MICROBES & INFECTIONS | 2016年 / 5卷

基金：

美国国家卫生研究院;

关键词：

Cambodia; epidemic; hand foot and mouth disease; human enterovirus 71; severe disease; ASIA-PACIFIC REGION; COXSACKIEVIRUS A6; SEQUENCE ALIGNMENT; MOSAIC STRUCTURE; RISK-FACTORS; CHILDREN; CHINA; TAIWAN; RECOMBINATION; EVOLUTION;

D O I：

10.1038/emi.2016.101

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human enterovirus 71 (EV-A71) causes hand, foot and mouth disease (HFMD). EV-A71 circulates in many countries and has caused large epidemics, especially in the Asia-Pacific region, since 1997. In April 2012, an undiagnosed fatal disease with neurological involvement and respiratory distress occurred in young children admitted to the Kantha Bopha Children's Hospital in Phnom Penh, Cambodia. Most died within a day of hospital admission, causing public panic and international concern. In this study, we describe the enterovirus (EV) genotypes that were isolated during the outbreak in 2012 and the following year. From June 2012 to November 2013, 312 specimens were collected from hospitalized and ambulatory patients and tested by generic EV and specific EV-A71 reverse transcription PCR. EV-A71 was detected in 208 clinical specimens while other EVs were found in 32 patients. The VP1 gene and/or the complete genome were generated. Our phylogenetic sequencing analysis demonstrated that 80 EV-A71 strains belonged to the C4a subgenotype and 3 EV-A71 strains belonged to the B5 genotype. Furthermore, some lineages of EV-A71 were found to have appeared in Cambodia following separate introductions from neighboring countries. Nineteen EV A (CV-A6 and CV-A16), 9 EV B (EV-B83, CV-B3, CV-B2, CV-A9, E-31, E-2 and EV-B80) and 4 EV C (EV-C116, EV-C96, CV-A20 and Vaccine-related PV-3) strains were also detected. We found no molecular markers of disease severity. We report here that EV-A71 genotype C4 was the main etiological agent of a large outbreak of HFMD and particularly of severe forms associated with central nervous system infections. The role played by other EVs in the epidemic could not be clearly established.

引用

页数：9

共 88 条

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