High-Throughput Single-Cell TCR-pMHC Dissociation Rate Measurements Performed by an Autonomous Microfluidic Cellular Processing Unit

被引：1
作者：
Jammes, Fabien [1 ]
Schmidt, Julien [2 ,3 ,4 ]
Coukos, George [2 ,3 ,4 ]
Maerkl, Sebastian J. [1 ]
机构：
[1] Ecole Polytech Fed Lausanne, Inst Bioengn, Sch Engn, CH-1015 Lausanne, Switzerland
[2] Ludwig Inst Canc Res, CH-1066 Lausanne, Switzerland
[3] Univ Lausanne, Dept Oncol, CH-1066 Lausanne, Switzerland
[4] Univ Hosp Lausanne CHUV, CH-1066 Lausanne, Switzerland
来源：
ACS SENSORS | 2022年 / 7卷 / 01期
关键词：
micro fl uidics; single-cell analysis; TCR; pMHC; immunotherapy; cancer; lab-on-chip; CULTURE;
D O I：
10.1021/acssensors.1c01935
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We developed an integrated microfluidic cellular processing unit (mCPU) capable of autonomously isolating single cells and performing measurements and on-the-fly analysis of cell- surface dissociation rates, followed by recovery of selected cells. We performed proof-of-concept, high-throughput single-cell experiments characterizing pMHC-TCR interactions on live CD8+ T cells. The mCPU platform analyzed TCR-pMHC dissociation rates with a throughput of 50 cells per hour and hundreds of cells per run, and we demonstrate that cells can be selected, enriched, and easily recovered from the device.
引用
收藏
页码：159 / 165
页数：7
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共 19 条
  • [1] TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency
    Allard, Mathilde
    Couturaud, Barbara
    Carretero-Iglesia, Laura
    Minh Ngoc Duong
    Schmidt, Julien
    Monnot, Gwennaelle C.
    Romero, Pedro
    Speiser, Daniel E.
    Hebeisen, Michael
    Rufer, Nathalie
    [J]. JCI INSIGHT, 2017, 2 (14):
  • [2] Versatile, fully automated, microfluidic cell culture system
    Gomez-Sjoeberg, Rafael
    Leyrat, Anne A.
    Pirone, Dana M.
    Chen, Christopher S.
    Quake, Stephen R.
    [J]. ANALYTICAL CHEMISTRY, 2007, 79 (22) : 8557 - 8563
  • [3] Identifying Individual T Cell Receptors of Optimal Avidity for Tumor Antigens
    Hebeisen, Michael
    Allard, Mathilde
    Gannon, Philippe O.
    Schmidt, Julien
    Speiser, Daniel E.
    Rufer, Nathalie
    [J]. FRONTIERS IN IMMUNOLOGY, 2015, 6 : 1 - 18
  • [4] Identification of Rare High-Avidity, Tumor-Reactive CD8+ T Cells by Monomeric TCR-Ligand Off-Rates Measurements on Living Cells
    Hebeisen, Michael
    Schmidt, Julien
    Guillaume, Philippe
    Baumgaertner, Petra
    Speiser, Daniel E.
    Luescher, Immanuel
    Rufer, Nathalie
    [J]. CANCER RESEARCH, 2015, 75 (10) : 1983 - 1991
  • [5] Interplay between T Cell Receptor Binding Kinetics and the Level of Cognate Peptide Presented by Major Histocompatibility Complexes Governs CD8+ T Cell Responsiveness
    Irving, Melita
    Zoete, Vincent
    Hebeisen, Michael
    Schmid, Daphne
    Baumgartner, Petra
    Guillaume, Philippe
    Romero, Pedro
    Speiser, Daniel
    Luescher, Immanuel
    Rufer, Nathalie
    Michielin, Olivier
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (27) : 23068 - 23078
  • [6] How single-cell immunology is benefiting from microfluidic technologies
    Jammes, Fabien C.
    Maerkl, Sebastian J.
    [J]. MICROSYSTEMS & NANOENGINEERING, 2020, 6 (01)
  • [7] Integrated platform for cell culture and dynamic quantification of cell secretion
    Kaestli, Alicia J.
    Junkin, Michael
    Tay, Savas
    [J]. LAB ON A CHIP, 2017, 17 (23) : 4124 - 4133
  • [8] TCR-pMHC kinetics under force in a cell-free system show no intrinsic catch bond, but a minimal encounter duration before binding
    Limozin, Laurent
    Bridge, Marcus
    Bongrand, Pierre
    Dushek, Omer
    van der Merwe, Philip Anton
    Robert, Philippe
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (34) : 16943 - 16948
  • [9] Single-cell analysis and sorting using droplet-based microfluidics
    Mazutis, Linas
    Gilbert, John
    Ung, W. Lloyd
    Weitz, David A.
    Griffiths, Andrew D.
    Heyman, John A.
    [J]. NATURE PROTOCOLS, 2013, 8 (05) : 870 - 891
  • [10] TCR-Ligand koff Rate Correlates with the Protective Capacity of Antigen-Specific CD8+ T Cells for Adoptive Transfer
    Nauerth, Magdalena
    Weissrich, Bianca
    Knall, Robert
    Franz, Tobias
    Doessinger, Georg
    Bet, Jeannette
    Paszkiewicz, Paulina J.
    Pfeifer, Lukas
    Bunse, Mario
    Uckert, Wolfgang
    Holtappels, Rafaela
    Gillert-Marien, Dorothea
    Neuenhahn, Michael
    Krackhardt, Angela
    Reddehase, Matthias J.
    Riddell, Stanley R.
    Busch, Dirk H.
    [J]. SCIENCE TRANSLATIONAL MEDICINE, 2013, 5 (192)
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