Immunodetection of the replicative complex of Barley yellow dwarf virus-PAV in vivo

被引：3
|
作者：
Fomitcheva, VW [1 ]
Schubert, J [1 ]
Rabenstein, F [1 ]
Habekuss, A [1 ]
机构：
[1] Fed Ctr Breeding Res Cultivated Plants, Inst Epidemiol & Resistance, D-06449 Aschersleben, Germany
来源：
EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2005年 / 112卷 / 03期
关键词：
Barley yellow dwarf virus; RNA-dependent RNA polymerase; replication; serology;
D O I：
10.1007/s10658-005-2082-2
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The genomic fragments of two open reading frames (ORFs) 1 and 2 of German and Canadian PAV isolates of Barley yellow dwarf virus (BYDV-PAV) were sequenced. Sequences only slightly differed from previously published sequences of this virus. Two polyclonal antisera against proteins encoded by ORFs 1 and 2 of a German ASL-1 isolate were developed using recombinant antigens expressed in E. coli as a fusion either to His(6)- or thioredoxin-tags. In Western blot analysis with total protein extracts from BYDV infected plants, antisera efficiently recognized the 99 kDa fusion protein expressed from ORF1 and ORF2 (P1-P2 protein). Later in infection the P1-P2 protein disappeared and two smaller proteins, revealing sizes of 39 and 60 kDa, could be detected.
引用
收藏
页码：259 / 266
页数：8
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